U3: DiATOME Diamond Knives, Knife Cleaning, Cryo Tools & Heat Pen
DiATOME DIAMOND KNIVES
ProSciTech is an agent for DiATOME. We supply these top quality diamond knives at excellent prices. Why purchase a second-rating knife and hassle with a greater chance of a warranty return, more waiting periods and a lower service life of an inferior knife? Send an email and ask for price and availability. Price includes freight into your store. We accept purchase orders from Universities and other large organisations or credit card purchases.
Unique characteristics
Manual for diamond knife handling and use
Guarantee
Before delivery, each knife, new or resharpened, is subject to extensive testing. Perfect sectioning (in the respective thickness range) over the entire cutting edge is guaranteed. The knives are guaranteed for an unlimited number of resharpenings.
Resharpened diamond knives
Any DiATOME knife can be resharpened an unlimited number of times. Resharpened DiATOME knives undergo the same stringent optical checking and sectioning tests as new knives. A diamond knife resharpened by DiATOME is the same high quality as a new one.
Your resharpened DiATOME knife will have the same length, the identical cutting edge and carry the same guarantee of quality as the day it first left the factory.
Damaged or discoloured boats will be replaced at no charge.
Note for overseas customers: If you send us a diamond knife for resharpening, you must make the Custom's Declaration: No commercial value. Returning for repair. Otherwise, you will be charged the duty payable on the knife.
Resharpened diamond knife with exchange
Rather than having your own knife resharpened, the knife can be exchanged for an already resharpened knife. In the exchange service a type change is also possible (ultra to cryo, 45° to 35°, small knives to large ones, etc). This includes changing the boat to your desired style.
Custom diamond knives and boats
Upon request, we can supply different coloured (black) and shaped boats (Huxley for instance) at no extra cost. Certain applications require special knives and/or tools, outside the standard knives and tools available. For years DiATOME has been manufacturing custom diamond blades, tools and knives for customers, meeting all of their specific needs. There is no charge for this service, only the regular cost of the knife once you have approved it.
Delivery time and shipping
All new knives as well as exchange knives are available for delivery in 2 weeks. For our resharpening service, the turn-around time is no longer than 6 weeks from date that the knife is received by us. All of our knives, new or resharpened, are shipped at our expense.
More DiATOME programs and options
ProSciTech is an agent for DiATOME. We supply these top quality diamond knives at excellent prices. Why purchase a second-rating knife and hassle with a greater chance of a warranty return, more waiting periods and a lower service life of an inferior knife? Send an email and ask for price and availability. Price includes freight into your store. We accept purchase orders from Universities and other large organisations or credit card purchases.
Unique characteristics
- DiATOME offers incomparable durability of the diamond edge. DiATOME knives have been known to last for many years without resharpening.
- All DiATOME diamonds are of the highest possible quality with a regular crystal structure. The crystal orientation of the diamond permits a final polishing parallel to the cutting edge, which gives a perfect nick free edge.
- Uniform sections are easily reproducible.
- DiATOME knives are guaranteed for an unlimited number of resharpenings.
- When we resharpen our diamond knives, we restore them to their original condition. Your resharpened DiATOME diamond knife will have the same length, the identical cutting edge and carry the same guarantee of quality as the day it first left our factory.
- Our diamonds are fastened into a metal shank which is securely screwed into the boat and then sealed. The sealing material is hydrophilic, allowing for easy wetting of the cutting edge, and has no effect on the stability of the knife.
- For our cryo knives we use a special epoxy resin for sealing which is chemical and temperature resistant.
- Before delivery each knife, new or resharpened, is subjected to extensive testing for its ability to cut accurately without scoring or compression. Only if its performance passes our tests will we ship it to you.
- The DiATOME boat is designed in such a manner that the water surface is horizontal when the clearance angle is set. This results in stationary water surface and good reflection.
- DiATOME's universal knife boats are designed to fit all brands of ultramicrotomes without the need for an adapter.
- The shape of the DiATOME boat offers the advantage of easy pick-up of the floating sections.
| Knife | Angle | Sizes | Cutting Range | Application |
|---|---|---|---|---|
| Ultra | 35° | 1.5-4.0mm | 30-150 nm | Standard applications |
| 1.5-4.0mm | 10-30 nm | Electron Spectroscopic Imaging (ESI), 3D Reconstruction, etc. | ||
| 1.5-4.0mm | 50nm - 1µ | Cryo sectioning | ||
| 1.5-4.0mm | 100nm-2µm | Alternating ultra-thin/semi-thin sectioning | ||
| Ultra | 45° | 1.5-4.0mm | 30-200 nm | Standard applications |
| 1.5-4.0mm | 50nm-1µ | Alternating ultra-thin/semi-thin sectioning | ||
| Ultra Jumbo | 35° | 2.0, 3.0mm | For longer section ribbons | |
| 45° | 2.0, 3.0mm | For longer section ribbons | ||
| Ultra AFM | 35° | 2.0, 3.0mm | 10-100nm | Surface sectioning for biological and material specimens for AFM investigation |
| Ultra Sonic | 35° | 3.0mm | 10nm-80nm | Ultra thin sections without compression |
| Cryo Dry | 25° | 3.0mm | 25nm-200nm | Frozen hydrated specimens |
| Cryo Immuno | 35° | 2.0, 3.0mm | 30nm-1µm | Cryo-immuno-chemistry |
| Cryo AFM | 35° | 2.0, 3.0mm | 10-100nm | Surface sectioning for biological and material specimens for AFM investigation |
| Cryo Dry | 35° | 1.5-4.0mm | 30nm-1µ | Frozen biological and material samples such as polymers, rubbers and paints |
| 45° | 1.5-4.0mm | 30nm-1µ | Routine cryo sectioning | |
| Cryo Wet | 35° | 1.5-4.0mm | 30nm-1µ | Frozen biological and material samples such as polymers, rubbers and paints |
| 45° | 1.5-4.0mm | 30nm-1µ | Routine cryo sectioning | |
| Cryotrim 20 | 20° | Trimming of sucrose protected as well as frozen hydrated biological samples and polymers | ||
| Cryotrim 45 | 45° | Trimming of sucrose protected as well as frozen hydrated biological samples and polymers | ||
| Ultratrim wet or dry | 3.0, 4.0mm | Trimming of epoxy and acrylic embeddings as well as polymers and non-ferrous metals |
||
| Histo | 45° | 4.0, 6.0, 8.0mm | 0.2-5µm | Sectioning of biological and material specimens non embedded or embedded (plastic or epoxy) for light microscopy |
| Histo Cryo Wet | 45° | 4.0, 6.0mm | 0.2-5µm | Cryo sectioning with a boat for light microscopy |
| Histo Cryo Dry | 45° | 4.0, 6.0mm | 0.2-5µm | Cryo Sectioning with a triangular holder for light microscopy |
| Histo Jumbo | 45° | 6.0, 8.0mm | 0.2-5µm | Immuno-histo-chemistry and 3D reconstruction |
| Static Line II | Reduction of static charging. |
Manual for diamond knife handling and use
Guarantee
Before delivery, each knife, new or resharpened, is subject to extensive testing. Perfect sectioning (in the respective thickness range) over the entire cutting edge is guaranteed. The knives are guaranteed for an unlimited number of resharpenings.
Resharpened diamond knives
Any DiATOME knife can be resharpened an unlimited number of times. Resharpened DiATOME knives undergo the same stringent optical checking and sectioning tests as new knives. A diamond knife resharpened by DiATOME is the same high quality as a new one.
Your resharpened DiATOME knife will have the same length, the identical cutting edge and carry the same guarantee of quality as the day it first left the factory.
Damaged or discoloured boats will be replaced at no charge.
Note for overseas customers: If you send us a diamond knife for resharpening, you must make the Custom's Declaration: No commercial value. Returning for repair. Otherwise, you will be charged the duty payable on the knife.
Resharpened diamond knife with exchange
Rather than having your own knife resharpened, the knife can be exchanged for an already resharpened knife. In the exchange service a type change is also possible (ultra to cryo, 45° to 35°, small knives to large ones, etc). This includes changing the boat to your desired style.
Custom diamond knives and boats
Upon request, we can supply different coloured (black) and shaped boats (Huxley for instance) at no extra cost. Certain applications require special knives and/or tools, outside the standard knives and tools available. For years DiATOME has been manufacturing custom diamond blades, tools and knives for customers, meeting all of their specific needs. There is no charge for this service, only the regular cost of the knife once you have approved it.
Delivery time and shipping
All new knives as well as exchange knives are available for delivery in 2 weeks. For our resharpening service, the turn-around time is no longer than 6 weeks from date that the knife is received by us. All of our knives, new or resharpened, are shipped at our expense.
More DiATOME programs and options
THE ROOM TEMPERATURE ULTRA-THIN COLLECTION
The Ultra knives are available with 35 and 45 degree angles. The optimal thickness range for cutting with these knives is between 30 - 150nm. Sizes range from 1.5 - 4.0mm as a standard. Other sizes are available upon request. All of the knives are tested within a thickness range of 30 - 150nm. Whether your needs are biological or materials related, in EM or LM, we have a knife to suit your needs.
Ultra 45°
Recognised as the knife for routine sectioning. It is a good compromise between section quality and durability.
The 45° knife is recommended for the sectioning of extremely hard and brittle materials such as ceramics. In this domain it has replaced the formerly recommended 55° knife.
Ultra 35°
The capabilities of the 35° diamond knives have been shown in two publications by J.Jésior (Ref 3, 4). He proved markedly reduced compression, smoother section surfaces and better structural preservation with the use of our 35° diamond knives.
In the meantime a great number of scientists have noticed and recognised the advantages of the 35° knives for the sectioning of Lowicryls, inhomogenous samples such as undecalcified bone, dental materials, etc.
The 35° knives have proved advantageous in the sectioning of soft industrial samples such as metals and polymers, as well as for hard and brittle samples, for example semiconductors (Si, GaAs, etc), superconducting oxides, nanocrystalline ceramics.
The use of 35° knives leads to reduced compression in soft samples and to less breaking in hard and brittle samples.
DiATOME ULTRA DIAMOND KNIVES, 35°
UU35-15 Diamond knife, Ultra, 35°, 1.5 - 1.7mm, new ask Availability
UU35-15E Diamond knife, Ultra, 35°, 1.5 - 1.7mm, exchanged ask
UU35-15R Diamond knife, Ultra, 35°, 1.5 - 1.7mm, resharpened ask
UU35-18 Diamond knife, Ultra, 35°, 1.8 - 2.0mm, new ask Availability
UU35-18E Diamond knife, Ultra, 35°, 1.8 - 2.0mm, exchanged ask
UU35-18R Diamond knife, Ultra, 35°, 1.8 - 2.0mm, resharpened ask
UU35-21 Diamond knife, Ultra, 35°, 2.1 - 2.3mm, new ask Availability
UU35-21E Diamond knife, Ultra, 35°, 2.1 - 2.3mm, exchanged ask
UU35-21R Diamond knife, Ultra, 35°, 2.1 - 2.3mm, resharpened ask
UU35-24 Diamond knife, Ultra, 35°, 2.4 - 2.6mm, new ask Availability
UU35-24E Diamond knife, Ultra, 35°, 2.4 - 2.6mm, exchanged ask
UU35-24R Diamond knife, Ultra, 35°, 2.4 - 2.6mm, resharpened ask
UU35-25 Diamond knife, Ultra, 35°, 2.5 - 2.7mm, new ask Availability
UU35-25E Diamond knife, Ultra, 35°, 2.5 - 2.7mm, exchanged ask
UU35-25R Diamond knife, Ultra, 35°, 2.5 - 2.7mm, resharpened ask
UU35-30 Diamond knife, Ultra, 35°, 3.0 - 3.4mm, new ask Availability
UU35-30E Diamond knife, Ultra, 35°, 3.0 - 3.4mm, exchanged ask
UU35-30R Diamond knife, Ultra, 35°, 3.0 - 3.4mm, resharpened ask
UU35-35 Diamond knife, Ultra, 35°, 3.5 - 3.9mm, new ask Availability
UU35-35E Diamond knife, Ultra, 35°, 3.5 - 3.9mm, exchanged ask
UU35-35R Diamond knife, Ultra, 35°, 3.5 - 3.9mm, resharpened ask
UU35-40 Diamond knife, Ultra, 35°, 4.0 - 4.4mm, new ask Availability
UU35-40E Diamond knife, Ultra, 35°, 4.0 - 4.4mm, exchanged ask
UU35-40R Diamond knife, Ultra, 35°, 4.0 - 4.4mm, resharpened ask
UU35-15 Diamond knife, Ultra, 35°, 1.5 - 1.7mm, new ask Availability
UU35-15E Diamond knife, Ultra, 35°, 1.5 - 1.7mm, exchanged ask
UU35-15R Diamond knife, Ultra, 35°, 1.5 - 1.7mm, resharpened ask
UU35-18 Diamond knife, Ultra, 35°, 1.8 - 2.0mm, new ask Availability
UU35-18E Diamond knife, Ultra, 35°, 1.8 - 2.0mm, exchanged ask
UU35-18R Diamond knife, Ultra, 35°, 1.8 - 2.0mm, resharpened ask
UU35-21 Diamond knife, Ultra, 35°, 2.1 - 2.3mm, new ask Availability
UU35-21E Diamond knife, Ultra, 35°, 2.1 - 2.3mm, exchanged ask
UU35-21R Diamond knife, Ultra, 35°, 2.1 - 2.3mm, resharpened ask
UU35-24 Diamond knife, Ultra, 35°, 2.4 - 2.6mm, new ask Availability
UU35-24E Diamond knife, Ultra, 35°, 2.4 - 2.6mm, exchanged ask
UU35-24R Diamond knife, Ultra, 35°, 2.4 - 2.6mm, resharpened ask
UU35-25 Diamond knife, Ultra, 35°, 2.5 - 2.7mm, new ask Availability
UU35-25E Diamond knife, Ultra, 35°, 2.5 - 2.7mm, exchanged ask
UU35-25R Diamond knife, Ultra, 35°, 2.5 - 2.7mm, resharpened ask
UU35-30 Diamond knife, Ultra, 35°, 3.0 - 3.4mm, new ask Availability
UU35-30E Diamond knife, Ultra, 35°, 3.0 - 3.4mm, exchanged ask
UU35-30R Diamond knife, Ultra, 35°, 3.0 - 3.4mm, resharpened ask
UU35-35 Diamond knife, Ultra, 35°, 3.5 - 3.9mm, new ask Availability
UU35-35E Diamond knife, Ultra, 35°, 3.5 - 3.9mm, exchanged ask
UU35-35R Diamond knife, Ultra, 35°, 3.5 - 3.9mm, resharpened ask
UU35-40 Diamond knife, Ultra, 35°, 4.0 - 4.4mm, new ask Availability
UU35-40E Diamond knife, Ultra, 35°, 4.0 - 4.4mm, exchanged ask
UU35-40R Diamond knife, Ultra, 35°, 4.0 - 4.4mm, resharpened ask
DiATOME ULTRA DIAMOND KNIVES, 45°
UU45-15 Diamond knife, Ultra, 45°, 1.5 - 1.7mm, new ask Availability
UU45-15E Diamond knife, Ultra, 45°, 1.5 - 1.7mm, exchanged ask
UU45-15R Diamond knife, Ultra, 45°, 1.5 - 1.7mm, resharpened ask
UU45-18 Diamond knife, Ultra, 45°, 1.8 - 2.0mm, new ask Availability
UU45-18E Diamond knife, Ultra, 45°, 1.8 - 2.0mm, exchanged ask
UU45-18R Diamond knife, Ultra, 45°, 1.8 - 2.0mm, resharpened ask
UU45-21 Diamond knife, Ultra, 45°, 2.1 - 2.3mm, new ask Availability
UU45-21E Diamond knife, Ultra, 45°, 2.1 - 2.3mm, exchanged ask
UU45-21R Diamond knife, Ultra, 45°, 2.1 - 2.3mm, resharpened ask
UU45-24 Diamond knife, Ultra, 45°, 2.4 - 2.6mm, new ask Availability
UU45-24E Diamond knife, Ultra, 45°, 2.4 - 2.6mm, exchanged ask
UU45-24R Diamond knife, Ultra, 45°, 2.4 - 2.6mm, resharpened ask
UU45-25 Diamond knife, Ultra, 45°, 2.5 - 2.7mm, new ask Availability
UU45-25E Diamond knife, Ultra, 45°, 2.5 - 2.7mm, exchanged ask
UU45-25R Diamond knife, Ultra, 45°, 2.5 - 2.7mm, resharpened ask
UU45-30 Diamond knife, Ultra, 45°, 3.0 - 3.4mm, new ask Availability
UU45-30E Diamond knife, Ultra, 45°, 3.0 - 3.4mm, exchanged ask
UU45-30R Diamond knife, Ultra, 45°, 3.0 - 3.4mm, resharpened ask
UU45-35 Diamond knife, Ultra, 45°, 3.5 - 3.9mm, new ask Availability
UU45-35E Diamond knife, Ultra, 45°, 3.5 - 3.9mm, exchanged ask
UU45-35R Diamond knife, Ultra, 45°, 3.5 - 3.9mm, resharpened ask
UU45-40 Diamond knife, Ultra, 45°, 4.0 - 4.4mm, new ask Availability
UU45-40E Diamond knife, Ultra, 45°, 4.0 - 4.4mm, exchanged ask
UU45-40R Diamond knife, Ultra, 45°, 4.0 - 4.4mm, resharpened ask
UU45-15 Diamond knife, Ultra, 45°, 1.5 - 1.7mm, new ask Availability
UU45-15E Diamond knife, Ultra, 45°, 1.5 - 1.7mm, exchanged ask
UU45-15R Diamond knife, Ultra, 45°, 1.5 - 1.7mm, resharpened ask
UU45-18 Diamond knife, Ultra, 45°, 1.8 - 2.0mm, new ask Availability
UU45-18E Diamond knife, Ultra, 45°, 1.8 - 2.0mm, exchanged ask
UU45-18R Diamond knife, Ultra, 45°, 1.8 - 2.0mm, resharpened ask
UU45-21 Diamond knife, Ultra, 45°, 2.1 - 2.3mm, new ask Availability
UU45-21E Diamond knife, Ultra, 45°, 2.1 - 2.3mm, exchanged ask
UU45-21R Diamond knife, Ultra, 45°, 2.1 - 2.3mm, resharpened ask
UU45-24 Diamond knife, Ultra, 45°, 2.4 - 2.6mm, new ask Availability
UU45-24E Diamond knife, Ultra, 45°, 2.4 - 2.6mm, exchanged ask
UU45-24R Diamond knife, Ultra, 45°, 2.4 - 2.6mm, resharpened ask
UU45-25 Diamond knife, Ultra, 45°, 2.5 - 2.7mm, new ask Availability
UU45-25E Diamond knife, Ultra, 45°, 2.5 - 2.7mm, exchanged ask
UU45-25R Diamond knife, Ultra, 45°, 2.5 - 2.7mm, resharpened ask
UU45-30 Diamond knife, Ultra, 45°, 3.0 - 3.4mm, new ask Availability
UU45-30E Diamond knife, Ultra, 45°, 3.0 - 3.4mm, exchanged ask
UU45-30R Diamond knife, Ultra, 45°, 3.0 - 3.4mm, resharpened ask
UU45-35 Diamond knife, Ultra, 45°, 3.5 - 3.9mm, new ask Availability
UU45-35E Diamond knife, Ultra, 45°, 3.5 - 3.9mm, exchanged ask
UU45-35R Diamond knife, Ultra, 45°, 3.5 - 3.9mm, resharpened ask
UU45-40 Diamond knife, Ultra, 45°, 4.0 - 4.4mm, new ask Availability
UU45-40E Diamond knife, Ultra, 45°, 4.0 - 4.4mm, exchanged ask
UU45-40R Diamond knife, Ultra, 45°, 4.0 - 4.4mm, resharpened ask
DiATOME ULTRA JUMBO DIAMOND KNIVES
The Ultra diamond knife is now available in Jumbo with 35° and 45° angles and in 2.0mm and 3.0mm sizes. The larger boat accommodates long ribbons for serial sections or when searching for a particular structure.
UUJ35-20 Diamond knife, Ultra Jumbo, 35°, 2.0 - 2.3mm, new ask Availability
UUJ35-20R Diamond knife, Ultra Jumbo, 35°, 2.0 - 2.3mm, resharpened ask
UUJ35-30 Diamond knife, Ultra Jumbo, 35°, 3.0 - 3.4mm, new ask Availability
UUJ35-30R Diamond knife, Ultra Jumbo, 35°, 3.0 - 3.4mm, resharpened ask
UUJ45-20 Diamond knife, Ultra Jumbo, 45°, 2.0 - 2.3mm, new ask Availability
UUJ45-20R Diamond knife, Ultra Jumbo, 45°, 2.0 - 2.3mm, resharpened ask
UUJ45-30 Diamond knife, Ultra Jumbo, 45°, 3.0 - 3.4mm, new ask Availability
UUJ45-30R Diamond knife, Ultra Jumbo, 45°, 3.0 - 3.4mm, resharpened ask
The Ultra diamond knife is now available in Jumbo with 35° and 45° angles and in 2.0mm and 3.0mm sizes. The larger boat accommodates long ribbons for serial sections or when searching for a particular structure.
UUJ35-20 Diamond knife, Ultra Jumbo, 35°, 2.0 - 2.3mm, new ask Availability
UUJ35-20R Diamond knife, Ultra Jumbo, 35°, 2.0 - 2.3mm, resharpened ask
UUJ35-30 Diamond knife, Ultra Jumbo, 35°, 3.0 - 3.4mm, new ask Availability
UUJ35-30R Diamond knife, Ultra Jumbo, 35°, 3.0 - 3.4mm, resharpened ask
UUJ45-20 Diamond knife, Ultra Jumbo, 45°, 2.0 - 2.3mm, new ask Availability
UUJ45-20R Diamond knife, Ultra Jumbo, 45°, 2.0 - 2.3mm, resharpened ask
UUJ45-30 Diamond knife, Ultra Jumbo, 45°, 3.0 - 3.4mm, new ask Availability
UUJ45-30R Diamond knife, Ultra Jumbo, 45°, 3.0 - 3.4mm, resharpened ask
LOW TEMPERATURE ULTRA-THIN COLLECTION
Cryo sectioning for electron microscopy is performed in cryo-ultramicrotomes, generally at -100°C, which prevents ice crystal formation. Prior to sectioning, biological specimens are vitrified by most rapid freezing to about -160°C. Cryo diamond knives are indispensible tools in this technically complex procedure.
Available for either wet or dry sectioning with a 25°, 35° or a 45° angle.
Section thickness range 30nm - 1µm.
Cutting edge sizes 1.5 - 4mm.
DiATOME Cryo diamond knives may be used for all low temperature situations, such as sectioning of cryo-protected specimens, frozen hydrated specimens and industrial samples such as polymers and rubber.
There are numerous advantages to our Cryo diamond knives compared with glass knives:
Cryo 25°
The Cryo 25° knife is designed for sectioning frozen hydrated specimens. The 25° angle results in the least possible compression and the best structure preservation.
Cryo 35° and Cryo 45°
The 45° knife is well suited for routine cryo sectioning.
The 35° knife allows sectioning with considerable reduction of mechanical damage to the specimen (up to 20% less compression as compared to the 45° knife) and, in turn, better structural preservation of most frozen samples (Ref 6, 7, 8).
The triangular holder for dry sectioning and the boat for the sectioning with a liquid (DMSO/water and others) are made of a special copper/nickel alloy. They both guarantee excellent cold or heat transmission.
The seal is made of a cold-resistant epoxy resin.
More information about DiATOME Cryo knives
Cryo sectioning for electron microscopy is performed in cryo-ultramicrotomes, generally at -100°C, which prevents ice crystal formation. Prior to sectioning, biological specimens are vitrified by most rapid freezing to about -160°C. Cryo diamond knives are indispensible tools in this technically complex procedure.
Available for either wet or dry sectioning with a 25°, 35° or a 45° angle.
Section thickness range 30nm - 1µm.
Cutting edge sizes 1.5 - 4mm.
DiATOME Cryo diamond knives may be used for all low temperature situations, such as sectioning of cryo-protected specimens, frozen hydrated specimens and industrial samples such as polymers and rubber.
There are numerous advantages to our Cryo diamond knives compared with glass knives:
- They produce thinner sections
- Ultrathin and semithin sections with the same knife
- Easy to produce long ribbons of sections
- Less compression and greater uniformity of sections
- Time saving. No knife manufacturing, no knife changing, hence no waiting for temperature equilibration.
- Diamond is the best temperature conductor - better than gold. No waiting for temperature equilibration
Cryo 25°
The Cryo 25° knife is designed for sectioning frozen hydrated specimens. The 25° angle results in the least possible compression and the best structure preservation.
Cryo 35° and Cryo 45°
The 45° knife is well suited for routine cryo sectioning.
The 35° knife allows sectioning with considerable reduction of mechanical damage to the specimen (up to 20% less compression as compared to the 45° knife) and, in turn, better structural preservation of most frozen samples (Ref 6, 7, 8).
The triangular holder for dry sectioning and the boat for the sectioning with a liquid (DMSO/water and others) are made of a special copper/nickel alloy. They both guarantee excellent cold or heat transmission.
The seal is made of a cold-resistant epoxy resin.
More information about DiATOME Cryo knives
DiATOME CRYO DRY DIAMOND KNIVES, 25° and 35°
UCD25-30 Diamond knife, Cryo Dry, 25°, 3.0 - 3.4mm, new ask Availability
UCD25-30R Diamond knife, Cryo Dry, 25°, 3.0 - 3.4mm, resharpened ask
UCD35-15 Diamond knife, Cryo Dry, 35°, 1.5 - 1.7mm, new ask Availability
UCD35-15E Diamond knife, Cryo Dry, 35°, 1.5 - 1.7mm, exchanged ask
UCD35-15R Diamond knife, Cryo Dry, 35°, 1.5 - 1.7mm, resharpened ask
UCD35-18 Diamond knife, Cryo Dry, 35°, 1.8 - 2.0mm, new ask Availability
UCD35-18E Diamond knife, Cryo Dry, 35°, 1.8 - 2.0mm, exchanged ask
UCD35-18R Diamond knife, Cryo Dry, 35°, 1.8 - 2.0mm, resharpened ask
UCD35-21 Diamond knife, Cryo Dry, 35°, 2.1 - 2.3mm, new ask Availability
UCD35-21E Diamond knife, Cryo Dry, 35°, 2.1 - 2.3mm, exchanged ask
UCD35-21R Diamond knife, Cryo Dry, 35°, 2.1 - 2.3mm, resharpened ask
UCD35-24 Diamond knife, Cryo Dry, 35°, 2.4 - 2.6mm, new ask Availability
UCD35-24E Diamond knife, Cryo Dry, 35°, 2.4 - 2.6mm, exchanged ask
UCD35-24R Diamond knife, Cryo Dry, 35°, 2.4 - 2.6mm, resharpened ask
UCD35-25 Diamond knife, Cryo Dry, 35°, 2.5 - 2.7mm, new ask Availability
UCD35-25E Diamond knife, Cryo Dry, 35°, 2.5 - 2.7mm, exchanged ask
UCD35-25R Diamond knife, Cryo Dry, 35°, 2.5 - 2.7mm, resharpened ask
UCD35-30 Diamond knife, Cryo Dry, 35°, 3.0 - 3.4mm, new ask Availability
UCD35-30E Diamond knife, Cryo Dry, 35°, 3.0 - 3.4mm, exchanged ask
UCD35-30R Diamond knife, Cryo Dry, 35°, 3.0 - 3.4mm, resharpened ask
UCD35-35 Diamond knife, Cryo Dry, 35°, 3.5 - 3.9mm, new ask Availability
UCD35-35E Diamond knife, Cryo Dry, 35°, 3.5 - 3.9mm, exchanged ask
UCD35-35R Diamond knife, Cryo Dry, 35°, 3.5 - 3.9mm, resharpened ask
UCD35-40 Diamond knife, Cryo Dry, 35°, 4.0 - 4.4mm, new ask Availability
UCD35-40E Diamond knife, Cryo Dry, 35°, 4.0 - 4.4mm, exchanged ask
UCD35-40R Diamond knife, Cryo Dry, 35°, 4.0 - 4.4mm, resharpened ask
UCD25-30 Diamond knife, Cryo Dry, 25°, 3.0 - 3.4mm, new ask Availability
UCD25-30R Diamond knife, Cryo Dry, 25°, 3.0 - 3.4mm, resharpened ask
UCD35-15 Diamond knife, Cryo Dry, 35°, 1.5 - 1.7mm, new ask Availability
UCD35-15E Diamond knife, Cryo Dry, 35°, 1.5 - 1.7mm, exchanged ask
UCD35-15R Diamond knife, Cryo Dry, 35°, 1.5 - 1.7mm, resharpened ask
UCD35-18 Diamond knife, Cryo Dry, 35°, 1.8 - 2.0mm, new ask Availability
UCD35-18E Diamond knife, Cryo Dry, 35°, 1.8 - 2.0mm, exchanged ask
UCD35-18R Diamond knife, Cryo Dry, 35°, 1.8 - 2.0mm, resharpened ask
UCD35-21 Diamond knife, Cryo Dry, 35°, 2.1 - 2.3mm, new ask Availability
UCD35-21E Diamond knife, Cryo Dry, 35°, 2.1 - 2.3mm, exchanged ask
UCD35-21R Diamond knife, Cryo Dry, 35°, 2.1 - 2.3mm, resharpened ask
UCD35-24 Diamond knife, Cryo Dry, 35°, 2.4 - 2.6mm, new ask Availability
UCD35-24E Diamond knife, Cryo Dry, 35°, 2.4 - 2.6mm, exchanged ask
UCD35-24R Diamond knife, Cryo Dry, 35°, 2.4 - 2.6mm, resharpened ask
UCD35-25 Diamond knife, Cryo Dry, 35°, 2.5 - 2.7mm, new ask Availability
UCD35-25E Diamond knife, Cryo Dry, 35°, 2.5 - 2.7mm, exchanged ask
UCD35-25R Diamond knife, Cryo Dry, 35°, 2.5 - 2.7mm, resharpened ask
UCD35-30 Diamond knife, Cryo Dry, 35°, 3.0 - 3.4mm, new ask Availability
UCD35-30E Diamond knife, Cryo Dry, 35°, 3.0 - 3.4mm, exchanged ask
UCD35-30R Diamond knife, Cryo Dry, 35°, 3.0 - 3.4mm, resharpened ask
UCD35-35 Diamond knife, Cryo Dry, 35°, 3.5 - 3.9mm, new ask Availability
UCD35-35E Diamond knife, Cryo Dry, 35°, 3.5 - 3.9mm, exchanged ask
UCD35-35R Diamond knife, Cryo Dry, 35°, 3.5 - 3.9mm, resharpened ask
UCD35-40 Diamond knife, Cryo Dry, 35°, 4.0 - 4.4mm, new ask Availability
UCD35-40E Diamond knife, Cryo Dry, 35°, 4.0 - 4.4mm, exchanged ask
UCD35-40R Diamond knife, Cryo Dry, 35°, 4.0 - 4.4mm, resharpened ask
DiATOME CRYO DRY DIAMOND KNIVES, 45°
UCD45-15 Diamond knife, Cryo Dry, 45°, 1.5 - 1.7mm, new ask Availability
UCD45-15E Diamond knife, Cryo Dry, 45°, 1.5 - 1.7mm, exchanged ask
UCD45-15R Diamond knife, Cryo Dry, 45°, 1.5 - 1.7mm, resharpened ask
UCD45-18 Diamond knife, Cryo Dry, 45°, 1.8 - 2.0mm, new ask Availability
UCD45-18E Diamond knife, Cryo Dry, 45°, 1.8 - 2.0mm, exchanged ask
UCD45-18R Diamond knife, Cryo Dry, 45°, 1.8 - 2.0mm, resharpened ask
UCD45-21 Diamond knife, Cryo Dry, 45°, 2.1 - 2.3mm, new ask Availability
UCD45-21E Diamond knife, Cryo Dry, 45°, 2.1 - 2.3mm, exchanged ask
UCD45-21R Diamond knife, Cryo Dry, 45°, 2.1 - 2.3mm, resharpened ask
UCD45-24 Diamond knife, Cryo Dry, 45°, 2.4 - 2.6mm, new ask Availability
UCD45-24E Diamond knife, Cryo Dry, 45°, 2.4 - 2.6mm, exchanged ask
UCD45-24R Diamond knife, Cryo Dry, 45°, 2.4 - 2.6mm, resharpened ask
UCD45-25 Diamond knife, Cryo Dry, 45°, 2.5 - 2.7mm, new ask Availability
UCD45-25E Diamond knife, Cryo Dry, 45°, 2.5 - 2.7mm, exchanged ask
UCD45-25R Diamond knife, Cryo Dry, 45°, 2.5 - 2.7mm, resharpened ask
UCD45-30 Diamond knife, Cryo Dry, 45°, 3.0 - 3.4mm, new ask Availability
UCD45-30E Diamond knife, Cryo Dry, 45°, 3.0 - 3.4mm, exchanged ask
UCD45-30R Diamond knife, Cryo Dry, 45°, 3.0 - 3.4mm, resharpened ask
UCD45-35 Diamond knife, Cryo Dry, 45°, 3.5 - 3.9mm, new ask Availability
UCD45-35E Diamond knife, Cryo Dry, 45°, 3.5 - 3.9mm, exchanged ask
UCD45-35R Diamond knife, Cryo Dry, 45°, 3.5 - 3.9mm, resharpened ask
UCD45-40 Diamond knife, Cryo Dry, 45°, 4.0 - 4.4mm, new ask Availability
UCD45-40E Diamond knife, Cryo Dry, 45°, 4.0 - 4.4mm, exchanged ask
UCD45-40R Diamond knife, Cryo Dry, 45°, 4.0 - 4.4mm, resharpened ask
UCD45-15 Diamond knife, Cryo Dry, 45°, 1.5 - 1.7mm, new ask Availability
UCD45-15E Diamond knife, Cryo Dry, 45°, 1.5 - 1.7mm, exchanged ask
UCD45-15R Diamond knife, Cryo Dry, 45°, 1.5 - 1.7mm, resharpened ask
UCD45-18 Diamond knife, Cryo Dry, 45°, 1.8 - 2.0mm, new ask Availability
UCD45-18E Diamond knife, Cryo Dry, 45°, 1.8 - 2.0mm, exchanged ask
UCD45-18R Diamond knife, Cryo Dry, 45°, 1.8 - 2.0mm, resharpened ask
UCD45-21 Diamond knife, Cryo Dry, 45°, 2.1 - 2.3mm, new ask Availability
UCD45-21E Diamond knife, Cryo Dry, 45°, 2.1 - 2.3mm, exchanged ask
UCD45-21R Diamond knife, Cryo Dry, 45°, 2.1 - 2.3mm, resharpened ask
UCD45-24 Diamond knife, Cryo Dry, 45°, 2.4 - 2.6mm, new ask Availability
UCD45-24E Diamond knife, Cryo Dry, 45°, 2.4 - 2.6mm, exchanged ask
UCD45-24R Diamond knife, Cryo Dry, 45°, 2.4 - 2.6mm, resharpened ask
UCD45-25 Diamond knife, Cryo Dry, 45°, 2.5 - 2.7mm, new ask Availability
UCD45-25E Diamond knife, Cryo Dry, 45°, 2.5 - 2.7mm, exchanged ask
UCD45-25R Diamond knife, Cryo Dry, 45°, 2.5 - 2.7mm, resharpened ask
UCD45-30 Diamond knife, Cryo Dry, 45°, 3.0 - 3.4mm, new ask Availability
UCD45-30E Diamond knife, Cryo Dry, 45°, 3.0 - 3.4mm, exchanged ask
UCD45-30R Diamond knife, Cryo Dry, 45°, 3.0 - 3.4mm, resharpened ask
UCD45-35 Diamond knife, Cryo Dry, 45°, 3.5 - 3.9mm, new ask Availability
UCD45-35E Diamond knife, Cryo Dry, 45°, 3.5 - 3.9mm, exchanged ask
UCD45-35R Diamond knife, Cryo Dry, 45°, 3.5 - 3.9mm, resharpened ask
UCD45-40 Diamond knife, Cryo Dry, 45°, 4.0 - 4.4mm, new ask Availability
UCD45-40E Diamond knife, Cryo Dry, 45°, 4.0 - 4.4mm, exchanged ask
UCD45-40R Diamond knife, Cryo Dry, 45°, 4.0 - 4.4mm, resharpened ask
DiATOME CRYO WET DIAMOND KNIVES, 35°
UCW35-15 Diamond knife, Cryo Wet, 35°, 1.5 - 1.7mm, new ask Availability
UCW35-15E Diamond knife, Cryo Wet, 35°, 1.5 - 1.7mm, exchanged ask
UCW35-15R Diamond knife, Cryo Wet, 35°, 1.5 - 1.7mm, resharpened ask
UCW35-18 Diamond knife, Cryo Wet, 35°, 1.8 - 2.0mm, new ask Availability
UCW35-18E Diamond knife, Cryo Wet, 35°, 1.8 - 2.0mm, exchanged ask
UCW35-18R Diamond knife, Cryo Wet, 35°, 1.8 - 2.0mm, resharpened ask
UCW35-21 Diamond knife, Cryo Wet, 35°, 2.1 - 2.3mm, new ask Availability
UCW35-21E Diamond knife, Cryo Wet, 35°, 2.1 - 2.3mm, exchanged ask
UCW35-21R Diamond knife, Cryo Wet, 35°, 2.1 - 2.3mm, resharpened ask
UCW35-24 Diamond knife, Cryo Wet, 35°, 2.4 - 2.6mm, new ask Availability
UCW35-24E Diamond knife, Cryo Wet, 35°, 2.4 - 2.6mm, exchanged ask
UCW35-24R Diamond knife, Cryo Wet, 35°, 2.4 - 2.6mm, resharpened ask
UCW35-25 Diamond knife, Cryo Wet, 35°, 2.5 - 2.7mm, new ask Availability
UCW35-25E Diamond knife, Cryo Wet, 35°, 2.5 - 2.7mm, exchanged ask
UCW35-25R Diamond knife, Cryo Wet, 35°, 2.5 - 2.7mm, resharpened ask
UCW35-30 Diamond knife, Cryo Wet, 35°, 3.0 - 3.4mm, new ask Availability
UCW35-30E Diamond knife, Cryo Wet, 35°, 3.0 - 3.4mm, exchanged ask
UCW35-30R Diamond knife, Cryo Wet, 35°, 3.0 - 3.4mm, resharpened ask
UCW35-35 Diamond knife, Cryo Wet, 35°, 3.5 - 3.9mm, new ask Availability
UCW35-35E Diamond knife, Cryo Wet, 35°, 3.5 - 3.9mm, exchanged ask
UCW35-35R Diamond knife, Cryo Wet, 35°, 3.5 - 3.9mm, resharpened ask
UCW35-40 Diamond knife, Cryo Wet, 35°, 4.0 - 4.4mm, new ask Availability
UCW35-40E Diamond knife, Cryo Wet, 35°, 4.0 - 4.4mm, exchanged ask
UCW35-40R Diamond knife, Cryo Wet, 35°, 4.0 - 4.4mm, resharpened ask
UCW35-15 Diamond knife, Cryo Wet, 35°, 1.5 - 1.7mm, new ask Availability
UCW35-15E Diamond knife, Cryo Wet, 35°, 1.5 - 1.7mm, exchanged ask
UCW35-15R Diamond knife, Cryo Wet, 35°, 1.5 - 1.7mm, resharpened ask
UCW35-18 Diamond knife, Cryo Wet, 35°, 1.8 - 2.0mm, new ask Availability
UCW35-18E Diamond knife, Cryo Wet, 35°, 1.8 - 2.0mm, exchanged ask
UCW35-18R Diamond knife, Cryo Wet, 35°, 1.8 - 2.0mm, resharpened ask
UCW35-21 Diamond knife, Cryo Wet, 35°, 2.1 - 2.3mm, new ask Availability
UCW35-21E Diamond knife, Cryo Wet, 35°, 2.1 - 2.3mm, exchanged ask
UCW35-21R Diamond knife, Cryo Wet, 35°, 2.1 - 2.3mm, resharpened ask
UCW35-24 Diamond knife, Cryo Wet, 35°, 2.4 - 2.6mm, new ask Availability
UCW35-24E Diamond knife, Cryo Wet, 35°, 2.4 - 2.6mm, exchanged ask
UCW35-24R Diamond knife, Cryo Wet, 35°, 2.4 - 2.6mm, resharpened ask
UCW35-25 Diamond knife, Cryo Wet, 35°, 2.5 - 2.7mm, new ask Availability
UCW35-25E Diamond knife, Cryo Wet, 35°, 2.5 - 2.7mm, exchanged ask
UCW35-25R Diamond knife, Cryo Wet, 35°, 2.5 - 2.7mm, resharpened ask
UCW35-30 Diamond knife, Cryo Wet, 35°, 3.0 - 3.4mm, new ask Availability
UCW35-30E Diamond knife, Cryo Wet, 35°, 3.0 - 3.4mm, exchanged ask
UCW35-30R Diamond knife, Cryo Wet, 35°, 3.0 - 3.4mm, resharpened ask
UCW35-35 Diamond knife, Cryo Wet, 35°, 3.5 - 3.9mm, new ask Availability
UCW35-35E Diamond knife, Cryo Wet, 35°, 3.5 - 3.9mm, exchanged ask
UCW35-35R Diamond knife, Cryo Wet, 35°, 3.5 - 3.9mm, resharpened ask
UCW35-40 Diamond knife, Cryo Wet, 35°, 4.0 - 4.4mm, new ask Availability
UCW35-40E Diamond knife, Cryo Wet, 35°, 4.0 - 4.4mm, exchanged ask
UCW35-40R Diamond knife, Cryo Wet, 35°, 4.0 - 4.4mm, resharpened ask
DiATOME CRYO WET DIAMOND KNIVES, 45°
UCW45-15 Diamond knife, Cryo Wet, 45°, 1.5 - 1.7mm, new ask Availability
UCW45-15E Diamond knife, Cryo Wet, 45°, 1.5 - 1.7mm, exchanged ask
UCW45-15R Diamond knife, Cryo Wet, 45°, 1.5 - 1.7mm, resharpened ask
UCW45-18 Diamond knife, Cryo Wet, 45°, 1.8 - 2.0mm, new ask Availability
UCW45-18E Diamond knife, Cryo Wet, 45°, 1.8 - 2.0mm, exchanged ask
UCW45-18R Diamond knife, Cryo Wet, 45°, 1.8 - 2.0mm, resharpened ask
UCW45-21 Diamond knife, Cryo Wet, 45°, 2.1 - 2.3mm, new ask Availability
UCW45-21E Diamond knife, Cryo Wet, 45°, 2.1 - 2.3mm, exchanged ask
UCW45-21R Diamond knife, Cryo Wet, 45°, 2.1 - 2.3mm, resharpened ask
UCW45-24 Diamond knife, Cryo Wet, 45°, 2.4 - 2.6mm, new ask Availability
UCW45-24E Diamond knife, Cryo Wet, 45°, 2.4 - 2.6mm, exchanged ask
UCW45-24R Diamond knife, Cryo Wet, 45°, 2.4 - 2.6mm, resharpened ask
UCW45-25 Diamond knife, Cryo Wet, 45°, 2.5 - 2.7mm, new ask Availability
UCW45-25E Diamond knife, Cryo Wet, 45°, 2.5 - 2.7mm, exchanged ask
UCW45-25R Diamond knife, Cryo Wet, 45°, 2.5 - 2.7mm, resharpened ask
UCW45-30 Diamond knife, Cryo Wet, 45°, 3.0 - 3.4mm, new ask Availability
UCW45-30E Diamond knife, Cryo Wet, 45°, 3.0 - 3.4mm, exchanged ask
UCW45-30R Diamond knife, Cryo Wet, 45°, 3.0 - 3.4mm, resharpened ask
UCW45-35 Diamond knife, Cryo Wet, 45°, 3.5 - 3.9mm, new ask Availability
UCW45-35E Diamond knife, Cryo Wet, 45°, 3.5 - 3.9mm, exchanged ask
UCW45-35R Diamond knife, Cryo Wet, 45°, 3.5 - 3.9mm, resharpened ask
UCW45-40 Diamond knife, Cryo Wet, 45°, 4.0 - 4.4mm, new ask Availability
UCW45-40E Diamond knife, Cryo Wet, 45°, 4.0 - 4.4mm, exchanged ask
UCW45-40R Diamond knife, Cryo Wet, 45°, 4.0 - 4.4mm, resharpened ask
UCW45-15 Diamond knife, Cryo Wet, 45°, 1.5 - 1.7mm, new ask Availability
UCW45-15E Diamond knife, Cryo Wet, 45°, 1.5 - 1.7mm, exchanged ask
UCW45-15R Diamond knife, Cryo Wet, 45°, 1.5 - 1.7mm, resharpened ask
UCW45-18 Diamond knife, Cryo Wet, 45°, 1.8 - 2.0mm, new ask Availability
UCW45-18E Diamond knife, Cryo Wet, 45°, 1.8 - 2.0mm, exchanged ask
UCW45-18R Diamond knife, Cryo Wet, 45°, 1.8 - 2.0mm, resharpened ask
UCW45-21 Diamond knife, Cryo Wet, 45°, 2.1 - 2.3mm, new ask Availability
UCW45-21E Diamond knife, Cryo Wet, 45°, 2.1 - 2.3mm, exchanged ask
UCW45-21R Diamond knife, Cryo Wet, 45°, 2.1 - 2.3mm, resharpened ask
UCW45-24 Diamond knife, Cryo Wet, 45°, 2.4 - 2.6mm, new ask Availability
UCW45-24E Diamond knife, Cryo Wet, 45°, 2.4 - 2.6mm, exchanged ask
UCW45-24R Diamond knife, Cryo Wet, 45°, 2.4 - 2.6mm, resharpened ask
UCW45-25 Diamond knife, Cryo Wet, 45°, 2.5 - 2.7mm, new ask Availability
UCW45-25E Diamond knife, Cryo Wet, 45°, 2.5 - 2.7mm, exchanged ask
UCW45-25R Diamond knife, Cryo Wet, 45°, 2.5 - 2.7mm, resharpened ask
UCW45-30 Diamond knife, Cryo Wet, 45°, 3.0 - 3.4mm, new ask Availability
UCW45-30E Diamond knife, Cryo Wet, 45°, 3.0 - 3.4mm, exchanged ask
UCW45-30R Diamond knife, Cryo Wet, 45°, 3.0 - 3.4mm, resharpened ask
UCW45-35 Diamond knife, Cryo Wet, 45°, 3.5 - 3.9mm, new ask Availability
UCW45-35E Diamond knife, Cryo Wet, 45°, 3.5 - 3.9mm, exchanged ask
UCW45-35R Diamond knife, Cryo Wet, 45°, 3.5 - 3.9mm, resharpened ask
UCW45-40 Diamond knife, Cryo Wet, 45°, 4.0 - 4.4mm, new ask Availability
UCW45-40E Diamond knife, Cryo Wet, 45°, 4.0 - 4.4mm, exchanged ask
UCW45-40R Diamond knife, Cryo Wet, 45°, 4.0 - 4.4mm, resharpened ask
THE HISTO KNIFE FOR LIGHT MICROSCOPY
For Room Temperature
Knives are available with a 45° angle.
Section thickness range 0.2 - 10µm.
Cutting edge length 4, 6, and 8mm.
The knife is designed for the sectioning of hard and soft biological and industrial materials, non- embedded or embedded in methacrylate or epoxy resins.
The Histo knife may be used on all ultramicrotomes and microtomes with a retraction of the specimen in the return phase.
For the production of our Histo knives we use natural diamonds of highest quality. They guarantee the best quality and durability of the cutting edges.
Some of the advantages of our Histo diamond knives compared with glass knives:
Histo Jumbo knives
For 3D reconstruction it is imperative not to lose a single section (Ref. Blumer).
The large Jumbo boat as well as the adhesive (Pattex compact by Henkel) applied to the side of the sample block increase the distinct advantages of our histo knives.
Features
For Cryo Temperatures
Knives are available with a 45° angle.
Section thickness range 0.2 - 5ìm.
Cutting edge length 4 and 6mm.
The Histo Cryo knives are delivered in boats (for wet cryo sectioning) and triangular holders (for dry cryo sectioning), made of a special copper/nickel alloy, which guarantees the best heat/cold transmission.
More information about DiATOME Histo knives
For Room Temperature
Knives are available with a 45° angle.
Section thickness range 0.2 - 10µm.
Cutting edge length 4, 6, and 8mm.
The knife is designed for the sectioning of hard and soft biological and industrial materials, non- embedded or embedded in methacrylate or epoxy resins.
The Histo knife may be used on all ultramicrotomes and microtomes with a retraction of the specimen in the return phase.
For the production of our Histo knives we use natural diamonds of highest quality. They guarantee the best quality and durability of the cutting edges.
Some of the advantages of our Histo diamond knives compared with glass knives:
- Perfect sections, free of scores or compression
- Serial sections without knife change
- Thinner sections
- Extreme durability of the cutting edge
- No knife making procedure
- Lifetime guarantee
Histo Jumbo knives
For 3D reconstruction it is imperative not to lose a single section (Ref. Blumer).
The large Jumbo boat as well as the adhesive (Pattex compact by Henkel) applied to the side of the sample block increase the distinct advantages of our histo knives.
Features
- Easy production of section ribbons (0.5-2µm)
- No section loss
- No folding
- The same orientation of all sections
- Easy collection of section ribbons
- Multiple ribbons on one glass slide
- Perfect for immuno-histo-chemistry
For Cryo Temperatures
Knives are available with a 45° angle.
Section thickness range 0.2 - 5ìm.
Cutting edge length 4 and 6mm.
The Histo Cryo knives are delivered in boats (for wet cryo sectioning) and triangular holders (for dry cryo sectioning), made of a special copper/nickel alloy, which guarantees the best heat/cold transmission.
More information about DiATOME Histo knives
Histo and Histo Jumbo
Histo Cryo Dry and Histo Cryo Wet
DiATOME HISTO KNIVES
UH45-40 Diamond knife, Histo, 45°, 4.0 - 4.9mm, new ask Availability
UH45-40E Diamond knife, Histo, 45°, 4.0 - 4.9mm, exchanged ask
UH45-40R Diamond knife, Histo, 45°, 4.0 - 4.9mm, resharpened ask
UH45-60 Diamond knife, Histo, 45°, 6.0 - 6.9mm, new ask Availability
UH45-60E Diamond knife, Histo, 45°, 6.0 - 6.9mm, exchanged ask
UH45-60R Diamond knife, Histo, 45°, 6.0 - 6.9mm, resharpened ask
UH45-80 Diamond knife, Histo, 45°, 8.0 - 8.9mm, new ask Availability
UH45-80E Diamond knife, Histo, 45°, 8.0 - 8.9mm, exchanged ask
UH45-80R Diamond knife, Histo, 45°, 8.0 - 8.9mm, resharpened ask
UH45-40 Diamond knife, Histo, 45°, 4.0 - 4.9mm, new ask Availability
UH45-40E Diamond knife, Histo, 45°, 4.0 - 4.9mm, exchanged ask
UH45-40R Diamond knife, Histo, 45°, 4.0 - 4.9mm, resharpened ask
UH45-60 Diamond knife, Histo, 45°, 6.0 - 6.9mm, new ask Availability
UH45-60E Diamond knife, Histo, 45°, 6.0 - 6.9mm, exchanged ask
UH45-60R Diamond knife, Histo, 45°, 6.0 - 6.9mm, resharpened ask
UH45-80 Diamond knife, Histo, 45°, 8.0 - 8.9mm, new ask Availability
UH45-80E Diamond knife, Histo, 45°, 8.0 - 8.9mm, exchanged ask
UH45-80R Diamond knife, Histo, 45°, 8.0 - 8.9mm, resharpened ask
DiATOME HISTO JUMBO KNIVES
UHJ45-60 Diamond knife, Histo Jumbo, 45°, 6.0 - 6.9mm, new ask Availability
UHJ45-60E Diamond knife, Histo Jumbo, 45°, 6.0 - 6.9mm, exchanged ask
UHJ45-60R Diamond knife, Histo Jumbo, 45°, 6.0 - 6.9mm, resharpened ask
UHJ45-80 Diamond knife, Histo Jumbo, 45°, 8.0 - 8.9mm, new ask Availability
UHJ45-80E Diamond knife, Histo Jumbo, 45°, 8.0 - 8.9mm, exchanged ask
UHJ45-80R Diamond knife, Histo Jumbo, 45°, 8.0 - 8.9mm, resharpened ask
UHJ45-60 Diamond knife, Histo Jumbo, 45°, 6.0 - 6.9mm, new ask Availability
UHJ45-60E Diamond knife, Histo Jumbo, 45°, 6.0 - 6.9mm, exchanged ask
UHJ45-60R Diamond knife, Histo Jumbo, 45°, 6.0 - 6.9mm, resharpened ask
UHJ45-80 Diamond knife, Histo Jumbo, 45°, 8.0 - 8.9mm, new ask Availability
UHJ45-80E Diamond knife, Histo Jumbo, 45°, 8.0 - 8.9mm, exchanged ask
UHJ45-80R Diamond knife, Histo Jumbo, 45°, 8.0 - 8.9mm, resharpened ask
DiATOME HISTO CRYO DRY KNIVES
UHCD45-40 Diamond knife, Histo Cryo Dry, 45°, 4.0 - 4.9mm, new ask Availability
UHCD45-40R Diamond knife, Histo Cryo Dry, 45°, 4.0 - 4.9mm, resharpened ask
UHCD45-60 Diamond knife, Histo Cryo Dry, 45°, 6.0 - 6.9mm, new ask Availability
UHCD45-60R Diamond knife, Histo Cryo Dry, 45°, 6.0 - 6.9mm, resharpened ask
UHCD45-40 Diamond knife, Histo Cryo Dry, 45°, 4.0 - 4.9mm, new ask Availability
UHCD45-40R Diamond knife, Histo Cryo Dry, 45°, 4.0 - 4.9mm, resharpened ask
UHCD45-60 Diamond knife, Histo Cryo Dry, 45°, 6.0 - 6.9mm, new ask Availability
UHCD45-60R Diamond knife, Histo Cryo Dry, 45°, 6.0 - 6.9mm, resharpened ask
DiATOME HISTO CRYO WET KNIVES
UHCW45-40 Diamond knife, Histo Cryo Wet, 45°, 4.0 - 4.9mm, new ask Availability
UHCW45-40R Diamond knife, Histo Cryo Wet, 45°, 4.0 - 4.9mm, resharpened ask
UHCW45-60 Diamond knife, Histo Cryo Wet, 45°, 6.0 - 6.9mm, new ask Availability
UHCW45-60R Diamond knife, Histo Cryo Wet, 45°, 6.0 - 6.9mm, resharpened ask
UHCW45-40 Diamond knife, Histo Cryo Wet, 45°, 4.0 - 4.9mm, new ask Availability
UHCW45-40R Diamond knife, Histo Cryo Wet, 45°, 4.0 - 4.9mm, resharpened ask
UHCW45-60 Diamond knife, Histo Cryo Wet, 45°, 6.0 - 6.9mm, new ask Availability
UHCW45-60R Diamond knife, Histo Cryo Wet, 45°, 6.0 - 6.9mm, resharpened ask
DiATOME CRYO IMMUNO DIAMOND KNIVES
The first cryo knife with a diamond platform guarantees the best possible sectioning for cryo-immuno-chemistry.
The diamond platform ensures easy and gentle section collection. Sections are collected directly from the diamond surface using a loop and a sucrose/methylcellulose droplet (Refs. Liou, Peters).
The 35° cutting angle leads to a considerable reduction in mechanical stresses and therefore to improved structure preservation in sucrose-protected samples.
More information about DiATOME Cryo Immuno knives
UCI35-20 Diamond knife, Cryo Immuno, 35°, 2.0mm, new ask Availability
UCI35-20R Diamond knife, Cryo Immuno, 35°, 2.0mm, resharpened ask
UCI35-30 Diamond knife, Cryo Immuno, 35°, 3.0mm, new ask Availability
UCI35-30R Diamond knife, Cryo Immuno, 35°, 3.0mm, resharpened ask
The first cryo knife with a diamond platform guarantees the best possible sectioning for cryo-immuno-chemistry.
The diamond platform ensures easy and gentle section collection. Sections are collected directly from the diamond surface using a loop and a sucrose/methylcellulose droplet (Refs. Liou, Peters).
The 35° cutting angle leads to a considerable reduction in mechanical stresses and therefore to improved structure preservation in sucrose-protected samples.
More information about DiATOME Cryo Immuno knives
UCI35-20 Diamond knife, Cryo Immuno, 35°, 2.0mm, new ask Availability
UCI35-20R Diamond knife, Cryo Immuno, 35°, 2.0mm, resharpened ask
UCI35-30 Diamond knife, Cryo Immuno, 35°, 3.0mm, new ask Availability
UCI35-30R Diamond knife, Cryo Immuno, 35°, 3.0mm, resharpened ask
Cryo Immuno
It is very important to remember that to successfully do dry cryosectioning you must eliminate electrostatic charges in the cryochamber. Our studies have shown that a single spike ioniser which produces a surplus of negative ions and is driven by high voltage is most effective in eliminating the electrostatic charges. Please see our section on THE STATIC LINE II IONISER.
DiATOME ULTRA SONIC DIAMOND KNIFE
The DiATOME ultra sonic, the oscillating diamond knife for room temperature ultramicrotomy, was developed in collaboration with Dr Daniel Studer, Lab. of Anatomy, University of Berne. A piezo actuator produces an oscillation of the knife at a desired frequency and amplitude, parallel with the cutting edge. A depression in the foot of the knife allows the oscillation parallel to the cutting edge. The depression is rigid in the north/south direction and guarantees stability in the cutting direction.
The knife produces ultrathin sections almost free of compression. The sections become thinner at the same thickness setting: since the volume of the section remains the same, the increased length leads to a decrease in thickness.
Specifications
Knife angle: 35º
Knife cutting range: 10-80nm
Knife cutting edge length: 3.0mm
Control Unit
Frequency: 25-45kHz, or automatic setting of the resonance
Amplitude: variable (Voltage 0-30V)
Mains voltage: 230V, 110V
More information about DiATOME Ultra Sonic knives
UUS35-30CU Diamond knife, Ultra Sonic, 35°, 3.0mm, including control unit ask Availability
UUS35-30 Diamond knife, Ultra Sonic, 35°, 3.0mm, new ask Availability
UUS35-30R Diamond knife, Ultra Sonic, 35°, 3.0mm, resharpened ask
The DiATOME ultra sonic, the oscillating diamond knife for room temperature ultramicrotomy, was developed in collaboration with Dr Daniel Studer, Lab. of Anatomy, University of Berne. A piezo actuator produces an oscillation of the knife at a desired frequency and amplitude, parallel with the cutting edge. A depression in the foot of the knife allows the oscillation parallel to the cutting edge. The depression is rigid in the north/south direction and guarantees stability in the cutting direction.
The knife produces ultrathin sections almost free of compression. The sections become thinner at the same thickness setting: since the volume of the section remains the same, the increased length leads to a decrease in thickness.
Specifications
Knife angle: 35º
Knife cutting range: 10-80nm
Knife cutting edge length: 3.0mm
Control Unit
Frequency: 25-45kHz, or automatic setting of the resonance
Amplitude: variable (Voltage 0-30V)
Mains voltage: 230V, 110V
More information about DiATOME Ultra Sonic knives
UUS35-30CU Diamond knife, Ultra Sonic, 35°, 3.0mm, including control unit ask Availability
UUS35-30 Diamond knife, Ultra Sonic, 35°, 3.0mm, new ask Availability
UUS35-30R Diamond knife, Ultra Sonic, 35°, 3.0mm, resharpened ask
UUS35-30CU
DiATOME AFM DIAMOND KNIVES, CRYO AND ULTRA
Extremely smooth sample surfaces and best possible structure preservation.
The high quality DiATOME diamond knives are not just perfect for producing ultrathin and semi-thin sections, but also for surface sectioning of all kinds of biological and industrial specimens for AFM investigation.
Instead of a section as for TEM, the specimen surface is investigated using AFM. In order to achieve the best results for AFM investigation, only the highest quality diamond knives should be used.
Diatome Ultra AFM and Cryo AFM knives are specially tested to ensure that they meet the increased quality requirements of AFM investigations. They produce extremely smooth sample surfaces and guarantee the best possible structure preservation.
Available with a 35° angle and a cutting range of 10-100nm, cutting edge length 2mm and 3mm.
More information about DiATOME AFM knives
UCA35-20 Diamond knife, Cryo AFM, 35°, 2.0mm, new ask Availability
UCA35-20R Diamond knife, Cryo AFM, 35°, 2.0mm, resharpened ask
UCA35-30 Diamond knife, Cryo AFM, 35°, 3.0mm, new ask Availability
UCA35-30R Diamond knife, Cryo AFM, 35°, 3.0mm, resharpened ask
UUA35-20 Diamond knife, Ultra AFM, 35°, 2.0mm, new ask Availability
UUA35-20R Diamond knife, Ultra AFM, 35°, 2.0mm, resharpened ask
UUA35-30 Diamond knife, Ultra AFM, 35°, 3.0mm, new ask Availability
UUA35-30R Diamond knife, Ultra AFM, 35°, 3.0mm, resharpened ask
Extremely smooth sample surfaces and best possible structure preservation.
The high quality DiATOME diamond knives are not just perfect for producing ultrathin and semi-thin sections, but also for surface sectioning of all kinds of biological and industrial specimens for AFM investigation.
Instead of a section as for TEM, the specimen surface is investigated using AFM. In order to achieve the best results for AFM investigation, only the highest quality diamond knives should be used.
Diatome Ultra AFM and Cryo AFM knives are specially tested to ensure that they meet the increased quality requirements of AFM investigations. They produce extremely smooth sample surfaces and guarantee the best possible structure preservation.
Available with a 35° angle and a cutting range of 10-100nm, cutting edge length 2mm and 3mm.
More information about DiATOME AFM knives
UCA35-20 Diamond knife, Cryo AFM, 35°, 2.0mm, new ask Availability
UCA35-20R Diamond knife, Cryo AFM, 35°, 2.0mm, resharpened ask
UCA35-30 Diamond knife, Cryo AFM, 35°, 3.0mm, new ask Availability
UCA35-30R Diamond knife, Cryo AFM, 35°, 3.0mm, resharpened ask
UUA35-20 Diamond knife, Ultra AFM, 35°, 2.0mm, new ask Availability
UUA35-20R Diamond knife, Ultra AFM, 35°, 2.0mm, resharpened ask
UUA35-30 Diamond knife, Ultra AFM, 35°, 3.0mm, new ask Availability
UUA35-30R Diamond knife, Ultra AFM, 35°, 3.0mm, resharpened ask
Cryo AFM and Ultra AFM
DIAMOND KNIFE CLEANING STICKS
High density polystyrene has been found most effective for cleaning of diamond knives. Instruction card is included.
User Notes - UD060
UD060 Diamond knife cleaning sticks AUD12.00 /pk/4 Available
High density polystyrene has been found most effective for cleaning of diamond knives. Instruction card is included.
User Notes - UD060
UD060 Diamond knife cleaning sticks AUD12.00 /pk/4 Available
UD060
STATIC LINE IONISER II
The Static Line II ioniser is an antistatic device which emits negative and positive ions, neutralising the electrostatic charging in the cryochamber.
The sections no longer stick to the knife edge, or bunch up one on top of the other, but float in a nice ribbon over the knife surface. The pickup of the sections can be done easily. The Static Line II ioniser is recommended for trimming, sectioning and section collection.
Range of applications
The Static Line II consists of a high voltage power pack, an ioniser electrode which is connected to the power pack by a cable, and a testing device, the MULTI CHECK.
A dial on the power pack allows for adjustment of the voltage. The correct ion emission can be achieved by turning the variable voltage dial.
Specifications
Voltage: 230 V, 115 V
Nominal frequency: 50-60Hz
Nominal output voltage: approx. 7-8 kV variable
Power consumption: approx. 50 VA
Short circuit output current: max. 3 mA
Dimensions: L173 x W113 x H110mm
Weight: approx. 3.5kg
U500 Static Line II ioniser ask Availability
The Static Line II ioniser is an antistatic device which emits negative and positive ions, neutralising the electrostatic charging in the cryochamber.
The sections no longer stick to the knife edge, or bunch up one on top of the other, but float in a nice ribbon over the knife surface. The pickup of the sections can be done easily. The Static Line II ioniser is recommended for trimming, sectioning and section collection.
Range of applications
- Sucrose protected specimens
- Cell cultures in elatine/ sucrose
- Frozen hydrated specimens
- Dry cut Lowicryls at room temperature
- Polymers and rubber
The Static Line II consists of a high voltage power pack, an ioniser electrode which is connected to the power pack by a cable, and a testing device, the MULTI CHECK.
A dial on the power pack allows for adjustment of the voltage. The correct ion emission can be achieved by turning the variable voltage dial.
Specifications
Voltage: 230 V, 115 V
Nominal frequency: 50-60Hz
Nominal output voltage: approx. 7-8 kV variable
Power consumption: approx. 50 VA
Short circuit output current: max. 3 mA
Dimensions: L173 x W113 x H110mm
Weight: approx. 3.5kg
U500 Static Line II ioniser ask Availability
U500
DiATOME CRYOTRIM TOOLS
For successful ultramicrotomy in biology and materials science, precise trimming is mandatory.
The DiATOME trimming blades Cryotrim 45 and Cryotrim 20 meet all trimming requirements, allowing quick, easy and accurate trimming.
Due to the extreme sharpness of diamond blades, less mechanical damage is applied to the sample during trimming. Very shiny sample faces and precise sides are the result.
The Cryotrim 45 produces pyramidal sides with an inclined angle of 45°, and the Cryotrim 20 produces pyramidal sides with an inclined angle of 20°.
UCT20 Cryotrim tool, 20°, new ask Availability
UCT20-R Cryotrim tool, 20°, resharpened ask
UCT45 Cryotrim tool, 45°, new ask Availability
UCT45-R Cryotrim tool, 45°, resharpened ask
For successful ultramicrotomy in biology and materials science, precise trimming is mandatory.
The DiATOME trimming blades Cryotrim 45 and Cryotrim 20 meet all trimming requirements, allowing quick, easy and accurate trimming.
Due to the extreme sharpness of diamond blades, less mechanical damage is applied to the sample during trimming. Very shiny sample faces and precise sides are the result.
The Cryotrim 45 produces pyramidal sides with an inclined angle of 45°, and the Cryotrim 20 produces pyramidal sides with an inclined angle of 20°.
UCT20 Cryotrim tool, 20°, new ask Availability
UCT20-R Cryotrim tool, 20°, resharpened ask
UCT45 Cryotrim tool, 45°, new ask Availability
UCT45-R Cryotrim tool, 45°, resharpened ask
Cryotrim tools
DiATOME ULTRATRIM TOOLS
Ultratrim for the trimming of epoxy and acrylic embeddings as well as polymers and non-ferrous metals is available either wet or dry and 3.0 and 4.0mm.
UUTD-30 Ultratrim tool, Dry, 3.0mm, new ask Availability
UUTD-30R Ultratrim tool, Dry, 3.0mm, resharpened ask
UUTD-40 Ultratrim tool, Dry, 4.0mm, new ask Availability
UUTD-40R Ultratrim tool, Dry, 4.0mm, resharpened ask
UUTW-30 Ultratrim tool, Wet, 3.0mm, new ask Availability
UUTW-30R Ultratrim tool, Wet, 3.0mm, resharpened ask
UUTW-40 Ultratrim tool, Wet, 4.0mm, new ask Availability
UUTW-40R Ultratrim tool, Wet, 4.0mm, resharpened ask
Ultratrim for the trimming of epoxy and acrylic embeddings as well as polymers and non-ferrous metals is available either wet or dry and 3.0 and 4.0mm.
UUTD-30 Ultratrim tool, Dry, 3.0mm, new ask Availability
UUTD-30R Ultratrim tool, Dry, 3.0mm, resharpened ask
UUTD-40 Ultratrim tool, Dry, 4.0mm, new ask Availability
UUTD-40R Ultratrim tool, Dry, 4.0mm, resharpened ask
UUTW-30 Ultratrim tool, Wet, 3.0mm, new ask Availability
UUTW-30R Ultratrim tool, Wet, 3.0mm, resharpened ask
UUTW-40 Ultratrim tool, Wet, 4.0mm, new ask Availability
UUTW-40R Ultratrim tool, Wet, 4.0mm, resharpened ask
Ultratrim Dry and Wet
HEAT PEN MAX WAX
For stretching of resin sections in ultramicrotomy and for making wax seals on glass knife troughs.
Its small, exposed heating element can reduce sectioning compression further than is possible using solvent vapour. It also reduces compression in semi-thin sections and eliminates the use of toxic chloroform vapour.
The element quickly forms wax seals around glass knives along the wax lined knife trough.
Two AA batteries power this model. The element is only heated when the switch is depressed; that is normally only for a couple of seconds, so batteries last a long time. In a very busy laboratory, these can be substituted with rechargeable batteries.
Replacement tips are available.
User Notes - U060
U060 Heat Pen for ultramicrotomy AUD48.00 Availability
U060R Heat pen replacement tip AUD12.00 10% discount for 3 Available
For stretching of resin sections in ultramicrotomy and for making wax seals on glass knife troughs.
Its small, exposed heating element can reduce sectioning compression further than is possible using solvent vapour. It also reduces compression in semi-thin sections and eliminates the use of toxic chloroform vapour.
The element quickly forms wax seals around glass knives along the wax lined knife trough.
Two AA batteries power this model. The element is only heated when the switch is depressed; that is normally only for a couple of seconds, so batteries last a long time. In a very busy laboratory, these can be substituted with rechargeable batteries.
Replacement tips are available.
User Notes - U060
U060 Heat Pen for ultramicrotomy AUD48.00 Availability
U060R Heat pen replacement tip AUD12.00 10% discount for 3 Available
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U060
U060R
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