S6: Counting Chambers, Haemacytometers/ Coverglass & Blood Diluting Pippettes
COUNTING CHAMBERS / HAEMACYTOMETERS
Principles and practice: for counting chambers
For cell counting, including 2 pieces of haemocytometer coverglass, without metal clamps, individually packed in plastic boxes.
These chambers are German made and thoroughly tested by the manufacturer. For an additional fee - and about 2 months lead time, we can supply gauged counting cells that have been certified by the German State Authority.
Helber Bacteria chamber - A one piece chamber with Thoma ruling on a single round plateau. The Helber Chamber is used for bacteria and sperm counting. Cell Depth is 0.02mm ± 1% (1/50mm). Volume of 1mm2: 0.02µL. BS748.
User note for Helber chamber
Malassez - This is a 2 cell counting chamber for haemacytes and leucocytes with a Malassez ruling 1/400mm2. Cell depth 0.2mm ± 1% (1/20mm). Volume of 1mm2: 0.2µL. Complete with 1 pair of cover glasses. This chamber is rhodium coated. Rectangular ruling covering an area of 5mm². Large rectangles measure 0.25 x 0.20mm (= 0.05mm²). They are each divided into 20 squares with an area of 0.0025mm².
Thoma-new - The small squares in the central large square measure 0.0025mm². As in the Thoma chamber the outer large squares are not completed. This chamber is used for counting thrombocytes and erythrocytes.
Nageotte - A two cell chamber for low leucocyte concentrations below the capabilities of a standard Haemacytometer. 40 rectangular areas in 0.25mm spaced vertical lines. The Nageotte can accurately measure down to 0.1 cell/mL (100 cell/mL). Cell Depth is 0.5mm ± 1% (1/2mm). Total Volume of 1mm2 of the grid is 0.5µL. This chamber is rhodium coated.
SVZ2NIOU Counting chamber Neubauer improved double net ruling AUD73.00 Available
SV150 Counting chamber Thoma double net ruling AUD70.00 Available
SV170 Counting chamber Thoma-New double net ruling AUD70.00 Available
SV190 Counting chamber Bürker double net ruling AUD70.00 Availability
SV210 Counting chamber Bürker-Türk double net ruling AUD70.00 Available
SV230 Counting chamber Malassez double net ruling AUD72.00 Available
SV250 Counting chamber Nageotte double net ruling AUD147.00 Available
SV270 Counting chamber Fuchs-Rosenthal double net ruling AUD73.00 Available
SV290 Counting chamber Schilling Kreutznetz double net ruling ask Availability
SV300 Counting chamber Schilling Einheitsnetz double net ruling AUD92.00 Available
SV310 Counting chamber Lemaur double net ruling AUD94.00 Available
SV320 Counting chamber Jessen double net ruling AUD92.00 Availability
SV330 Counting chamber Agasse-Lafont B double net ruling AUD92.00 Available
SV400 Counting chamber Helber bacteria, Thoma ruling AUD129.00 Available
COUNTING CHAMBERS, DOUBLE NET BRIGHT-LINED
As above counting chambers, but available with brightlines. These lines have a semi transparent metal coating, which makes these particularly useful under low contrast conditions.
SVZ4NIOU Counting chamber Neubauer improved double net ruling, bright-lined AUD105.00 Availability
SV151 Counting chamber Thoma double net ruling bright-lined AUD105.00 Available
SV171 Counting chamber Thoma-New double net ruling bright-lined AUD105.00 Available
SV191 Counting chamber Bürker double net ruling bright-lined AUD105.00 Available
SV211 Counting chamber Bürker-Türk double net ruling bright-lined AUD81.00 Available
SV231 Counting chamber Malassez double net ruling bright-lined AUD107.00 Available
SV251 Counting chamber Nageotte double net ruling bright-lined AUD130.00 Available
SV280 Counting chamber Ballast double net ruling bright-lined AUD213.00 Available
SV291 Counting chamber Schilling Kreutznetz double net ruling bright-lined AUD113.00 Available
SV301 Counting chamber Schilling Einheitsnetz double net ruling bright-lined AUD113.00 Availability
SV311 Counting chamber Lemaur double net ruling bright-lined AUD130.00 Available
SV321 Counting chamber Jessen double net ruling bright-lined AUD113.00 Available
SV341 Counting chamber Agasse-Lafont R double net ruling bright-lined AUD113.00 Available
SVZ4FROU Counting chamber Fuchs-Rosenthal double net ruling, bright-lined AUD106.00 Available
HAEMACYTOMETER SETS - Counting chamber Neubauer improved kit
These sets comprise of a cell counting chamber, cover slips, pipettes (red cell and white cell), tubing and pipette holders and instructions on usage and cleaning. Each set is packaged in a sturdy plastic box. The cell counting chamber is moulded from a single piece of thermal and shock-resistant glass. An H-shaped moat forms either one or two counting areas, or plateaus. Each plateau features an etched grid or ruling. The ruled surface is either 0.1mm or 0.2mm below the cover glass, limiting the volume of 1 square mm of blood or fluid over a square mm at 0.1cu.mm or 0.2cu.mm respectively. Contact of the flat, polished cover glass surfaces with the cover glass supports produces an exact volume of fluid over the counting area. The difference in surface tension characteristics between the metallic surface on the chamber and the polished cover glass assures smooth capillarity for precise loading and more even cell distribution.
2 cell standard - AS1000 + Z4A Improved NeubauerA 2 cell counting chamber set to BS748 with cell depth of 0.1mm and an Improved Neubauer Ruling.
2 cell rhodium coated - AC1000 + Z4 Improved NeubauerA 2 cell rhodium coated (bright-line) counting chamber set to BS748 with cell depth of 0.1mm and an Improved Neubauer Ruling.
SVZ2KIT Counting chamber Neubauer improved double net ruling AUD142.00 /kit Availability
SVZ4KIT Counting chamber Neubauer improved double net ruling, bright lined AUD155.00 /kit Availability
C-CHIP COUNTING CHAMBER, NEUBAUER IMPROVED, DISPOSABLE
The C-Chip is a disposable plastic haemocytometer used for manual cell counting. It consists of 2 enclosed, surface-patterned chambers with 2 ports for sample injection.
SpecificationsMeasurements: W25 x L75 x T1.6mm
Chamber volume: 10µL
Chamber depth: 0.1mm
SV520-N C-Chip counting chamber, Neubauer improved, disposable AUD158.00 /box/50 Availability
HAEMACYTOMETER/ COUNTING CHAMBER COVERGLASS
These glasses are thicker than standard coverglass to make them less flexible. They have also been flattened and polished to minimise variation that could change the depths of the counting chamber and affect accuracy of cell counts.
SVHA2026 Haemacytometer coverglass 20 x 26mm AUD12.00 /pk/10 Available
SVHC18 Coverglass for Helber chamber, Ø 18mm AUD17.00 /pk/10 Available
SVNC3232 Coverglass for Nageotte chamber, 32 x 32 x 0.1mm AUD63.00 /pack/2 Available
BLOOD DILUTING PIPETTES - Blood cell counter pipettes
Tubing and pipette holders
Tubing and mouthpieces for the red cell and white cell pipettes.
SVRED Red cell diluting pipette AUD20.00 Available
SVRM Tubing and mouthpiece for red cell pipettes AUD14.00 10% discount for 5 Available
SVWHITE White cell diluting pipette AUD21.00 Available
SVWM Tubing and mouthpiece for white cell pipettes AUD14.00 10% discount for 5 Available
S60 HOWARD CELL & K20 COVERGLASS - SV8060 & SV8020
User Notes - SV8060 and SV8020
The Howard cell, a 76mm x 35mm glass slide with a central circular island, is used for counting mould fibres and spores in fruit juices, especially from tomatoes. With the K20 coverglass in place a 0.1mm thickness of liquid is contained over the central island.
The coverglass has 25 calibrated fields of 1.382mm diameter through which to view the particles. This coverglass removes the necessity of precise adjustment of the microscope and eyepiece graticule in the original Howard method.
DUNN CHEMOTAXIS CHAMBER - SVDCC100
User Notes - SVDCC100
The Dunn chamber is a special, single round cell chambered slide. Cells are cultured on coverglass which is then inverted onto the slide. Cells resting over the annular bridge of the chamber can be observed under phase-contrast optics and their migration tracks are recorded automatically by time-lapse frame grabbing. Slide thickness 1mm, Dunn system, complete with 2 pieces of coverglass. BS748.Maybe autoclaved.
Cell depth: 0.02mm ± 1% (1/100mm)
Volume of 1 square mm: 0.02 microliter
Ruling pattern: none
McMASTER EGG COUNTING CHAMBER, 2 CELLS, ALL GLASS - SVZ11000
User Notes - SVZ11000
The McMaster egg counting chamber is used for determining faecal egg counts, which is a useful indicator of an animal's health. The slide is of all glass construction with the top covers affixed to the lower slide. Each of the two chambers has a 10mm x 10mm grid which is permanently etched on the under side of the top cover. Supplied complete with plain coverglass. Instructions included. BS748.
Use: Fecal egg counts
Cell depth: 1.5mm
Volume of 1 square mm: 0.15mL
Ruling pattern: 1mm squares, 1/100 square mm
McMASTER EGG COUNTING CHAMBER, 2 CELLS, GLASS AND PLASTIC - SVZ12000
This counting chamber has exactly the same grid and measurements between the upper and lower glass as the all glass version above, but the rest of the slide is constructed with a plastic bridge and ends. Can be sterilised by immersion in alcohol.
FERTILITY SEMEN COUNTING CHAMBER - SVZ3BC1B
User Notes - SVZ3BC1B
We supply Dewar vessels
Fertility semen counting chamber specifically designed for the counting of semen. A cell depth of 0.01mm is combined with a bright-line ruled 1mm square grid, divided into 100 equal squares. BS748.
Use: Semen counts
Cell depth: 0.01mm ± 1% (1/100mm)
Volume of 1 square mm: 0.1 microliter
Ruling pattern: 0.1mm x 0.1mm grid, 1/100 square mm
BACTERIA EVALUATION CHAMBER - SVZ80000
Single round cell. Slide 1mm thick. Standard optical platform. Complete with 2 pieces of coverglass. BS748.
Use: Bacteria motility and type evaluation
Cell depth: 0.02mm ± 1% (1/50mm)
Volume of 1 square mm: 0.02 microliter
Ruling pattern: none
SV8060 S60 Howard cell for mould counting AUD281.00 Available
SV8020 K20 calibrated coverglass for Howard cell AUD103.00 Available
SVDCC100 Dunn chemotaxis chamber AUD127.00 Availability
SVZ11000 McMaster egg counting chamber, 2 cells, all glass AUD224.00 Available
SVZ12000 McMaster egg counting chamber, 2 cells, glass and plastic AUD153.00 Available
SVZ3BC1B Fertility semen counting chamber AUD177.00 Available
SVZ80000 Bacteria evaluation chamber AUD96.00 Available
SEDGEWICK RAFTER COUNTING CELLS
These are used to count the number of cells, particles, or small organisms contained in a specified volume of fluid.
Coverglass for cells is included:
S8051 has a width of 30mm, length of 60mm and is 0.91mm thick.
S8050 and S8052 both come with coverglass S8051
S8050 S50 Sedgewick rafter counting cell, plastic AUD75.00 Available
S8051 Coverglass for Sedgewick rafter cells S50 and S52 AUD35.00 Available
S8052 S52 Sedgewick rafter counting cell, glass AUD229.00 Available
S8052R SR cell repair, regluing of frame to glass AUD88.00 Availability
UNIVERSAL WORM EGG COUNTING CHAMBER
The Universal worm egg counting chamber with 4 x 0.5mL cells, is specifically designed for parasite detection in large and small animals such as cattle, horses, sheep, goats, camels, deer, alpacas, ostriches, emus, dogs, cats and poultry. It is used by farmers, veterinarians, testing and research laboratories, teaching colleges and universities.
See also SVZ11000 Parasitology chamber improved McMaster 2 cell on this page.
The original McMaster counting chamber has 3 x 0.3mL cells and was designed for parasite detection in sheep and goats.
User Notes - S8060
S8060 Universal worm egg counting chamber AUD178.00 Available