We only stock the more common reticles and stage micrometers.
Our extensive range of counting cells and haemacytometers is on page S6.
Non stock item on this page are available within three weeks, except certified products which take longer.

CALIBRATION SCALES: STAGE MICROMETERS economy range
Stage micrometers are normally mounted on standard size slides. When the reticle image is on the glass surface, this micrometer can be brought into direct contact with the specimen mounted thereon for measurement. It is rarely practical or economical,however, to mount specimen and scale together. For this reason, most measurements in microscopy are made by means of an eyepiece reticle, and the function of the calibration scale is to calibrate the system. The stage micrometer is protected by a coverglass which also duplicates the specimen's optical conditions. The 1mm stage micrometer is also available without coverglass, for use with reflected light, as is commonly used in the material sciences.

Notes: Eyepiece reticles, stage micrometers & calibration factors

S8040 S1 stage micrometer with coverglass, 10mm x 0.1mm, transmitted light AUD185.00 Availability
S8041 S48 stage micrometer without coverglass, 1mm x 0.01mm, transmitted light AUD214.00 Availability
S8042 S8 stage micrometer with coverglass, 1mm x 0.01mm, transmitted light AUD185.00 10% discount for 10 Available
S8043 S78 stage micrometer without coverglass, 1mm x 0.1mm, reflected light AUD214.00 Availability
S8044 S12 stage micrometer with coverglass, 0.1mm x 0.002mm, transmitted light AUD288.00 Availability

CALIBRATION SCALES: STAGE MICROMETERS superior range
The following Stage Micrometers are supplied with the manufacturer's certificate of conformity. Also available, but at additional cost are Comparison (standards) Certificate by either the manufacturer or a choice of two independent Standards Laboratories. The glass discs are mounted in stainless steel slides with engraved serial numbers. Each slide is supplied in a polished wooden presentation and storage box to distinguish it as a traceable standard of high value.

S7PS12 Micrometer scale 0.1mm in 0.002mm divisions, w/certificate of conformity AUD435.00 Available
S7PS12-NPL S7PS12 with NPL certificate ask Availability
S7PS12-UKAS S7PS12 with UKAS certificate ask Availability
S7PS4 Micrometer scale 0.001 inch divisions, with certificate of conformity AUD307.00 Availability
S7PS78 Micrometer scale 1mm x 0.01mm, certificate of conformity, reflected light AUD307.00 Availability
S7PS78-NPL S7PS78 with NPL certificate ask Availability
S7PS78-UKAS S7PS78 with UKAS certificate AUD590.00 Availability
S7PS8 Micrometer scale 1mm in 0.01mm divisions, with certificate of conformity AUD307.00 Availability
S7PS8-NPL S7PS8 with NPL certificate ask Availability
S7PS8-UKAS S7PS8 with UKAS certificate AUD632.00 Availability

LONG STAGE MICROMETER FOR MICROSCOPE CALIBRATION
Best means for the calibrations of compound microscopes at low magnifications, stereo microscopes and measurement of fields of view that require a longer scale than those traditionally available. This new PS5 has a high accuracy scale of 20mm length , which is subdivided into 10 micrometer divisions.
The scale is produced as a vacuum deposited chrome image on a square of glass, with a coverglass cemented on top. This is mounted in a stainless steel slide mount and is supplied in a polished wooden case. Internationally traceable certificates of calibration are available to satisfy the requirements of ISO.

Calibration by NPL
The National Physical Laboratory carries out measurements at selected points on the scales and grids and issues a certificate of calibration.

Calibration by UKAS Accredited Laboratory
A UKAS accredited laboratory carries out measurements at selected points on the scales and grids and issues a calibration certificate.

Measurement by reticles
For applications which do not require the accuracy provided by calibrations carried out by NPL or a UKAS accredited laboratory, reticles can provide a Certificate of Comparison. The scale or grid is compared with NPL calibrated in-house standards and a statement is provided on the accuracy of the item with respect to these standards.

SPS5 S5 long stage micrometer, 20 x 0.01mm with certificate of conformity AUD356.00 Availability

Glass scales 50mm to 1000mm with 0.1mm divisions

STAGE MICROMETER
The dual circle with the scale centered in the inner circle is made from evaporated chromium. It is uncovered and therefore useable for reflected and transmitted light. If used to calibrate an oil immersion objective, it is preferable to mount a small circular cover slip on the stage micrometer slide.
We have tested the scale and it seems to be accurate to better than one percent; we cannot get it certified.
Clearly, accuracy is better than required for most biological applications and because of its low price, this calibration slide is suitable for school or university class exercises calibrating compound microscopes.

The scale divisions are 0.01mm and the total scale is 1mm long.
S81K Stage micrometer, glass, 1 x 0.01mm AUD33.00 10% discount for 5 Available
S81K-M Stage micrometer, metal, 1 x 0.01mm AUD85.00 10% discount for 5 Available

EYEPIECE RETICLES
Sizes other than the standard 16, 19 and 21mm
are available for an additional AUD55.00.

Note: Stage micrometers can be purchased certified, but not reticles. The area or length they 'see' would differ with the microscope and its configuration. So the readings are comparative unless used in conjunction with or calibrated using a stage micrometer. The comparative values can be entirely accurate.

Notes: Eyepiece reticles, stage micrometers & calibration factors (Calibration factor is essential for some reticles)

MICROMETERS
We offer a selected range of micrometer scales for the most popular applications in light microscopy. The reticles have surface patterns; sandwiched types are available at considerably greater cost.
E1 Horizontal Micrometers.
Length: 10mm, 100 divisions, 0.1mm per division.

Reticule Diameters for Common Microscopes

A Guide to Eyepiece Reticles

A Guide to Stage or Object Micrometers

S8010 NE1 Eyepiece graticule surface pattern 16mm diam AUD100.00 Available
S8011 NE1 Eyepiece graticule surface pattern 19mm diam AUD100.00 Available
S8012 NE1 Eyepiece graticule surface pattern 21mm diam AUD100.00 Available

BUDGET EYEPIECE RETICLE
The scale is 10mm long with numbered millimetres and scale graduated in tenth of millimeter.
The only size available is Ø 21mm, unless the minimum order is for 50 pieces or more.

S82-21 Budget eyepiece reticle, 10mm graduated line, Ø 21mm AUD27.00 10% discount for 10 Available

MICROMETERS WITH CROSSLINE
Length: 10mm; 100 divisions; 0.1mm per division.

S8013 NE7 micrometer w/crossline, 16mm AUD100.00 Availability
S8015 NE7 micrometer w/crossline, 21mm AUD100.00 Availability

SQUARE INDEXED RETICULES
Length of each grid square is 1.00mm.

S8016 NE11A reticule, square surface pattern 16mm AUD89.00 Availability
S8018 NE11A micrometer, reticule, square surface pattern 21mm AUD89.00 Available

CONCENTRIC CIRCLE MICROMETERS
Diameters: 0.25mm - 2.5mm

S8019 NE42 micrometer, concentric circles, 16mm AUD109.00 Available
S8021 NE42 micrometer, concentric circles, 21mm AUD109.00 Availability

FULL PROTRACTOR
Full protractor, scale 10mm diameter, divided in degrees. Surface chrome image.

S8031 NE45 full protractor, 16mm AUD109.00 Availability
S8032 NE45 full protractor, 19mm AUD109.00 Availability
S8033 NE45 full protractor, 21mm AUD109.00 Available

WEIBEL 1 RETICLES FOR STEREOLOGY
Composed of 15 equal lines which connect the vertices of a regular hexagonal point network. Weibel claims this pattern "ensures the end points are evenly distributed and avoids any bias." Ref.E.R Weibel, Lab. Invest., Vol. 22, pp131-155.
Principle and methods for the morphometric study of the lungs and other organs.

S8022 GW1 Weibel 1 graticule, 16mm AUD192.00 Availability
S8024 NGW1 Weibel 1 graticule, 21mm AUD192.00 Available

WEIBEL 2 RETICLES FOR STEREOLOGY
Used for the quantification of surface to volume ratio of structures per mass unit. This reticle has a number of short lines with equal short breaks. The number of intersections falling over the short lines are counted and the number of end points falling on the end of a structure is determined. Ref. E.R. Weibel, Journal of Microscopy, Vol.95, pp373-378.
Current capabilities and limitations of available stereological techniques: point counting method.

S8025 GW2 Weibel 2 graticule, 16mm AUD192.00 Availability
S8027 NGW2 Weibel 2 graticule, 21mm AUD192.00 Available

WALTON AND BECKETT RETICLES for particle fibre analysis
This reticle is used for counting fibrous dust, (e.g. asbestos or glass fibres) and is most useful where the majority of the fibres are smaller than 5 microns. The circle is divided into four by two diametrical lines scaled in units of 5 and 3 µm respectively. Unlike the usual globes of other partial reticles, Walton and Beckett's has a series of shapes for comparison. These shapes have been designed for comparison with fibres, especially since they incorporate an aspect ratio of 3:1 essential for such analysis. Important: for accurate work the counting field circle on this reticle must represent 100µm at the microscope stage. It is essential that the G22 suits a particular instrument and its configuration. When ordering a G22 reticle be sure to give details of your microscope, eyepiece, objective magnifications, and an exact calibration factor. Ref. Walton and Beckett, Ann. Occup. Hyg. Vol.20, 1977, pp. 19-23
A microscope eyepiece reticle for the evaluation of fibrous dusts.

Microscope for asbestos analysis on page O9

S8028 G22 Walton/Beckett graticule 16mm AUD217.00 Available
S8029 G22 Walton/Beckett graticule 19mm AUD217.00 Available
S8030 G22 Walton/Beckett graticule 21mm AUD217.00 Available
S8034 G22 Walton/Beckett graticule 26mm AUD217.00 Availability

ENGLAND FINDER COUNTING RETICLE SLIDE
The S7 England Finder is a glass slide with markings on the top surface. These markings give a reference position which is identical using any England Finder reticle. A grid pattern makes it possible to record positions.

User Notes - S8048

S8048 S7 England finder counting reticle AUD229.00 Available

HSL TEST SLIDE FOR CALIBRATION IN ASBESTOS ANALYSIS
The phase contrast test slide was developed by the Health and Safety Laboratory (HSL), on behalf of the Health and Safety Executive (HSE) and the National Physical Laboratory (NPL). It is made in the UK under licence from the National Physical Laboratory (NPL). Each of the HSE standard test slides is an epoxy replica of the NPL master, certified by HSL. The replica is mounted on a microscope slide and covered with a glass slip 0.17mm thick.
The test slide includes a calibration certificate by the British Health and Safety Laboratory, certifying that when viewed "by phase contrast optical microscopy, band 5 is fully visible and band 6 partially visible at 500x magnification."
Full details are supplied with the slide, more information can be found in the Specification & user notes link below.

Specifications & user notes

Note: Different test slides are in common use, or mandated by certifying authorities.
NIOSH (USA) red slide 4 bands.
HSE (UK and Australia) green slide 5 bands.
General non-asbestos microscope calibration yellow slide 6 bands.
USA may use band 5 and 4 if the calibrating person understands the process, similarly band 6 could also be used.

Walton & Beckett eyepiece reticles and PS12 calibration scales with UKAS certificates are frequently required by users of this slide - we stock both.

S8084-G HSE/ NPL test slide for calibration in asbestos analysis, 5 bands, green ask Availability

CALIBRATION STANDARD FOR MACHINE VISION AND IMAGE ANALYSIS SYSTEMS
Designed for measurement calibration of microscopes and machine vision systems.

Includes Concentric Circles and Squares, Line Gratings, Grid & Dot Arrays, Geometric root2 progression of Dots and Square blocks as well as course and variable fine linear Line Scales.

Each glass slide has a unique permanent serial number and can be supplied with full or partial UKAS certificate of accuracy.

Starting from a fixed ‘Datum point’ mark, each individual pattern or array can be located using X, Y coordinates. See User Note.

Calibration of microscopes and image analysis systems is becoming more sophisticated, with the requirement being for a variety of image patterns to satisfy the numerous parameters. Pyser-SGI has introduced a new multi-function calibration standard specifically for these applications.

Multiple images on a single slide provide the most cost-effective solution to calibration and resolution checking of microscopes and image analysis systems. The combination of scales, dots, circles, squares, rulings, grids and angles can be supplied with an internationally traceable certificate of calibration for those who require ISO conformity.

Specifications
General overall accuracy: 0.5µm
Coating: enduring evaporated chrome image
Optical density: >2.5
Substrate: soda lime glass
Size: 76 x 25 x 1.5mm
Packaging: polished wooden case

User note - Pattern description

SPS20 Universal calibration slide for machine vision, image analysis AUD437.00 Available

RONCHI RULING
This precision grating glass slide with 200 line-pairs/mm can be used with microscope or camera systems to check for resolution and distortion, and is supplied in a polished wood case for extra protection.
Metallic grating pattern with opaque lines. The glass is B270 with refractive index of 1.52.

Also available: ask
R12 - 2 line-pairs/mm
R15 - 8 line-pairs/mm
R16 - 20 line-pairs/mm
R17 - 40 line-pairs/mm
R25 - 50 line-pairs/mm
R26 - 100 line-pairs/mm
R27 - 125 line-pairs/mm

SR28 Ronchi ruling 200 line-pairs/mm AUD369.00 Available

IMPROVED USAF RESOLUTION TEST CHARTS
The original 1951 USAF Resolution Test Chart is a resolution test pattern which conforms to MIL-STD-150A standard, set by US Air Force in 1951. USAF resolution charts are recognised the world over as a universal standard for testing the vertical and horizontal resolution of imaging systems. Each element on the chart comprises three vertical bars and three horizontal bars; the resolution of the imaging system is normally specified as the finest Group and Element which can be clearly defined.

These four new versions with detail down to Group 9, Element 3, as fine as 0.78microns (644 line-pairs per mm, meet the need for higher resolution. Users can select positive or negative versions on either a square glass plate or steel microscope slide, to suit their application.

The slide version of the USAF resolution chart comes on a 76 x 25mm stainless steel mount to reduce the risk of breakage. A unique serial number is engraved in the surface of the mount to give traceability.

The square plate version is imaged on a glass plate measuring 50 x 50mm. This larger plate also has groups 0 and 1.

SPS75N USAF resolution chart, negative, groups 2/1 to 9/3, 76 x 25mm, steel AUD771.00 Availability
SPS75P USAF resolution chart, positive, groups 2/1 to 9/3, 76 x 25mm, steel AUD771.00 Availability
SR75N USAF resolution chart, negative, groups 0/1 to 9/3, 50 x 50mm, glass AUD882.00 Availability
SR75P USAF resolution chart, positive, groups 0/1 to 9/3, 50 x 50mm, glass AUD882.00 Availability

ORIGINAL USAF RESOLUTION CHARTS
Two patterns are available, 0 - 7 and -2 - 7, produced in accordance with the USAF Resolving Power Test Target 1951 Standard. The most popular range is 0-7 group, with 6 elements per group, an element consisting of two target patterns of 3 lines at 90 degrees to each other, the line space ratio being equal. These charts are produced as a chrome surface image on glass plate. The R70 has an image area of 12 x 12mm on a 51mm square plate and the R71 has an image area of 48 x 48mm on a 75mm square plate. Both plates are 1.5mm thick.

SR70 USAF resolution chart, groups 0 - 7, glass AUD178.00 Available
SR71 USAF resolution chart, groups -2 - 7, glass AUD246.00 Availability

PS52 CALIBRATION SLIDES, DUAL SCALE MM & INCH
Calibration slides with dual imperial/metric scales.The PS52P is for transmitted light applications and has a bright chrome positive image. The PS52N has a negative pattern, formed in low reflective chrome for incident light applications to give excellent contrast. Both are ideal for calibrating optical products with a large field of view, such as stereo microscopes  or imaging systems.

Features

• 50mm Metric scales and 2" imperial (English) on a single slide
• Positive and negative versions
• Unique serial number for traceability
• Available with internationally traceable certificates of calibration

Specifications

Metric scale	50mm in 0.1mm divisions
Imperial (English) scale	2" in 0.005" divisions
Line thickness	12 microns
Glass size/type	76mm x 25mm x 1.5mm, B270
Serial number	Unique serial number on slide surface
Case	Supplied in polished wooden box
Calibration certificate	Can be supplied with UKAS certificate of calibration which is internationally traceable and acceptable in all world markets

SPS52N Calibration slide, PS52N, dual scale, 50 x 0.1mm, negative AUD463.00 Availability
SPS52N-UKAS Calibration slide, PS52N, dual scale, negative, with UKAS certificate AUD855.00 Availability
SPS52P Calibration slide, PS52P, dual scale, 50 x 0.1mm, positive AUD463.00 Availability
SPS52P-UKAS Calibration slide, PS52P, dual scale, positive, with UKAS certificate AUD855.00 Availability

CALIBRATION SLIDES FOR HARDNESS TESTERS
PS25 is designed for Vickers and Rockwell methods and has a series of diamond shapes of varying size and x-y scales. Each of the markings on the slide is clearly identified with its size. The PS25 has a glass disc with the image precision marked in vacuum deposited chrome This is cemented into a stainless steel slide mount, making the item very durable. The slide has a unique serial number indelibly marked on the slide mount and can be supplied with an internationally traceable certificate of calibration.

PS26 is designed for the Brinell method and has a series of circles, to represent the ball indentation shape, of varying size and x-y scales. The PS26 has a precision marked chrome deposition image on a glass slide. Each of the markings on the slide is clearly identified with its size. The slide can be supplied with an internationally traceable certificate of calibration.

Both products are supplied in a polished wooden box.

Calibration is performed by a UKAS accredited laboratory, whose measurements are traceable back to the UK's National Metrology Institute, the National Physical Laboratory. All of the NMIs in the world work in harmony under the international diplomatic treaty, the Treaty of the Metre, signed in 1875. This means that all measurements carried out by the UKAS accredited laboratory are internationally traceable and satisfy the requirements of NIST, DIN and all other NMIs across the world.

SPS25 Calibration slide, PS25, for hardness testers, Vickers/Rockwell AUD308.00 Availability
SPS25-UKAS Calibration slide, PS25, for hardness testers, Vickers/Rockwell, with UKAS AUD913.00 Availability
SPS26 Calibration slide, PS26, for hardness testers, Brinell AUD308.00 Availability
SPS26-UKAS Calibration slide, PS26, for hardness testers, Brinell, with UKAS cert. AUD913.00 Availability