Tissue-Tek® O.C.T. COMPOUND cryostat specimen matrix
This is a much favoured water soluble glycols and resins compound that provides an excellent specimen matrix for cryostat sectioning at temperatures of -10^oC and below.
It leaves no residue during the staining procedure. MSDS

IA018 Tissue-Tek® O.C.T. Compound AUD35.00 /118mL 10% discount for 12 stock?

Tissue-Tack ADHESIVE
Improves permanent adhesion of sections to the glass slide during prolonged staining procedures and washes. Good for JB-4 and JB-4 Plus Sections.
Useful for immunofluorescent, immunoperoxidase and routine staining procedures. Store at RT.
User Notes

IM040 Tissue-Tack adhesive AUD105.00 /100mL stock?

Mounting media basics
The main purpose of mounting media is to physically protect the specimen; that also includes anti-fadent components for fluorescent dyes. Mounting media are usually applied as a couple of drops onto the specimen, which is then inverted with a hanging drop onto a coverslip. The mounting medium bonds specimen, slide and coverslip together with a clear durable film. The medium is important to the image formation as it affects the specimen's rendition. Until recently the most commonly used general mounting medium was DPX, this has been replaced by Entellan.

Mounting media are often chosen for a specific refractive index (R.I.), which can enhance specimen details or make them invisible. Materials like glass become totally invisible if immersed in a solution of the same refractive index. Glass chips can be identified by their R.I. All common mounting media use an R.I. close to that of borosilicate glass (coverslips), but a minor change of R.I. can improve the visibility of particular specimens. Most microscopists use a general mounting medium - like Entellan, but for special requirements like water-miscible, different R.I. or a need for an anti-fadent (especially in fluorescent microscopy), search for a special medium is advised.

Do not use immersion oil on an uncovered slide. If in a hurry to view a specimen under oil immersion, mount the slip and handle the mount carefully, keeping it horizontal. Oil can be applied, but do not attempt to wipe it off until the mounting medium is cured - at least overnight or an hour on a hotplate or in an oven. Do not store mounted slides vertically for 2 days if cured at room temperature.

Slides should be stored in a dark (slidebox) and cool place to slow fading of stains. Except for some special stains and mountants, mounting media and stains will last for decades and deterioration is very slow.

ENTELLAN® NEW
Replacing DPX, which is no longer made in Australia. Dissolved in xylene, a combination of several synthetic resins makes Entellan® New a particularly rapid embedding agent. It can be used for all dehydrated microscopic preparations. Embedded preparations can be filed for years without changes occurring in their colour. Constant properties ensure its high quality.
MSDS | User Notes

IM022 Entellan® New, mounting medium AUD37.00 /100mL stock?
IM0225 Entellan® New, mounting medium AUD155.00 /500mL stock?

CITIFLUOR™ ANTI-FADENT MOUNTING SOLUTIONS
These mounting media are designed to reduce the fading of the fluorescence of dyes used for labelling biological specimens.
In fluorescence microscopy, the fluorescence is stimulated by high intensity UV and/or visible light. Absorption of the light creates an excited state of the dye (usually a singlet state) and this leads to fluorescence. However, the excited state of the dye may undergo chemical reactions which lead to its destruction as evidenced by the fading or bleaching of the fluorescence and consequent loss of the image, or in the case of assays, a change in signal intensity during measurement.

There is no universal anti-fadent; due to the varied nature of biological material the fluorescence fading of fluorochromic conjugates is a complex process.
The general anti-fadents are AF1, AF2 and AF3. AF87 is a specialist immersion oil-based anti-fadent.

AF1 is an anti-fadent (anti-bleaching agent) contained in a glycerol PBS (phosphate-buffered saline) solution and is particularly useful for examining tissue sections.
AF2 solution contains the anti-fadent in glycerol which enables users to choose their own buffer.
AF3 which contains the anti-fadent in PBS solution, is particularly useful for examining live cells.
AF87 contains the anti-fadent in a nonfluorescent immersion oil. It is designed for those wanting to work at a high magnification. It is particularly useful for preventing the fading of such dyes as DAPI which have the unfortunate tendency to disperse in glycerol based mountant solutions.
CFPVOH is an aqueous solution of polyvinyl alcohol for use as a solid mountant. A few drops of the solution applied to your specimen and allowed to dry will produce a clear film.
AF100 is a solution of anti-fadents to be used with CFPVOH when fading of fluorescence in the polyvinyl alcohol is a problem. To prepare a CFPVOH solution containing AF100, add 0.1mL of AF100 to 0.9mL of CFPVOH. If more or less of the solid mountant solution is required, mix the reagents in the appropriate ratio. Once these solutions have been made up, they should be used within a period of days rather than months, since the solutions deteriorate on storage.

These products have a long shelf life, at least 2 years, and can be stored at room temperature. With an LD50 of >2000 mg/kg (rat) none of the solutions should pose a toxicological problem.
User Notes

IAF100S Set of Citifluor™ CFPVOH and AF100 anti-fadent AUD163.00 /25mL/5mL stock?
IAF1 Citifluor™ AF1 anti-fadent AUD142.00 /25mL stock?
IAF2 Citifluor™ AF2 anti-fadent AUD142.00 /25mL stock?
IAF3 Citifluor™ AF3 anti-fadent AUD142.00 /25mL stock?
IAF87 Citifluor™ AF87 anti-fadent AUD142.00 /10mL stock?

BIOMOUNT™ MOUNTING MEDIUM
Some mounting media oxidise rapidly upon exposure to air, forming carboxyl groups. This can be more pronounced when sections have been cleared in a solution containing aldehyde. It is sometimes observed that the immunogold/silver signal fades after a few weeks, or even in a shorter time, from sections that have been mounted with these media under cover slips. The silver is still present, but has formed translucent silver carboxylate salts. Visibility can be retrieved by removing the cover slip and washing in xylene and then immersing the slide in photographic developer, but this is a tedious procedure.
BioMount™ is a specially formulated mounting medium that reduces fading of immunogold/silver signals in sections on glass slides. It is suitable for resins as well as wax embedded sections of tissue. Store at RT. MSDS

IM028 BioMount™ mounting medium AUD92.00 /100mL stock?

CYTOSEAL™ MOUNTING MEDIUM
Premium quality; formulated from the highest quality advanced acrylic resin. It will not become brittle and crack, nor will it discolour or yellow with age. Cytoseal dries rapidly, allowing examination soon after application. It can be used with oil immersion objectives and in fluorescent procedures. Slides will not stick together and the medium will not "cold flow" to the edge of the slide during long term storage. CytoSeal can be dissolved in toluene or xylene. An antioxidant has been added to the formulation to prevent fading of slides. Cytoseal comes in a Controlled Drop Dispenser, with varying viscosities.

* Cytoseal™ 60, which has a viscosity of 60cps, is intended primarily for use with cover glass.
* Cytoseal™ 280, which has a viscosity of 280cps is intended for use where minimal spread of medium is required. MSDS

Both Cytoseal 60 and Cytoseal 280 have a refractive index of 1.48

IA013 Cytoseal™ 60 mounting medium AUD59.00 /120mL stock?
IA014 Cytoseal™ 280 mounting medium AUD89.00 /120mL stock?

CRYSTAL/MOUNT GEL/MOUNT MOUNTING MEDIA
These are non-hazardous water-based, mounting media which are ideal for mounting immunohistochemical stained tissues, which can be damaged or are soluble in organic solvents, such as xylene or toluene. Hence, tissue sections stained with chromagens such as 3-amino-9-ethyl carbazole and those using coupled dye systems with BCIP, naphthol-phosphate salts, etc. are best preserved using aqueous mounting media. Crystal/Mount allows for post-mounting of the specimens with Permount mounting medium. Gel/Mount is especially recommended for all fluorescence applications. Store at 0-5ºC.
User note for Gel/Mount
User note for Crystal/Mount

IM030 Biomeda Gel/Mount, water-based mounting medium AUD69.00 /20mL stock?
IM032 Biomeda Crystal/Mount, water-based mounting medium AUD88.00 /30mL stock?

SHUR/MOUNT
A Triangle Biomedical Sciences product. This is a fast drying acrylic resin with a low viscosity - which reduces air-bubbles. An antioxidant prevents fading of stains through years of storage and a UV absorbant prevents yellowing of resins. This mounting medium may be used as a cover itself, without a cover glass. As a mountant it is superior to most others. Supplied in a convenient twist-top plastic dispensing bottle that makes droppers redundant. For less rapid drying and for use in Cover Slipping Machines use the xylene based formulation. MSDS

IA017 SHUR/Mount, toluene-based mounting medium AUD77.00 /120mL 10% discount for 12 stock?

CANADA BALSAM
Natural resin, filtered. Well-tried mounting medium. Refractive index 1.52-1.54. Excellent for mounting cell smears and thick specimens. For this reason it is also preferred for mounting geological thin sections. When reheated, Canada Balsam mounted sections can be removed. This is useful for some operations like ion beam thinning. MSDS

IA158 Canada balsam AUD112.00 /100mL stock?
IA1585 Canada balsam AUD480.00 /500mL stock?

MELTMOUNT™ QUICK-STICK™ optical & SEM
Meltmount (optical quality) is a mounting medium formulated for use in microscope slide mounting and in other optical coupling applications.
Meltmount Features:

• Instant thermal mountants that require "no-oven-time".
• 100% usable; contains no solvents.
• Thermally, reversible for particle retrieval or re-orientation.
• Soluble in toluene for special techniques or clean-up.
• Fluid at 65^oC, a temperature that makes mounts "permanent" but protects the majority of specimens from thermal changes.
• The refractive index of this grade meltmount is nD @ 25^oC is 1.539 and the Abbe V dispersion is 45; This is a stock item.
• Meltmounts with the refractive indices of: 1.582; 1.605; 1.662; 1.680; 1.704 are available on indent at identical prices.
• A convenient replacement for other mounting media (Canada Balsam, Aroclor 5542, Naphrax...)
• Meltmount to match Chrysotile asbestos is also available at somewhat greater cost, ask.

Meltmount is supplied in an easy-to-use Quick-Stick™. Meltmount sticks can be applied to a slide on a hotplate. The slide is available for microscopy as soon as the specimen and coverglass are positioned and the slide has cooled. The coverglass of this "permanent" slide, however, can be lifted any time by reheating the slide. This makes it possible, for instance, to retrieve a particular particle. Each Quick-Stick weighs approximately 18g.
MSDS

Making microscope slide mounts using MELTMOUNT MOUNTING MEDIA

I011 Meltmount™ Quick-Stick™ mounting medium AUD77.00 stock?

EUKITT™ MOUNTING MEDIUM
Eukitt is a mounting medium (contains 45% acrylic resin and 55% xylenes), used in microscopy. It is an adhesive and specimen preservative that can be used manually and in automated coverslipping equipment. Special purpose adhesive and sealant for use with glass, non plastic and other non-reactive materials. It spreads quickly and evenly without forming any air bubbles.
Properties: Fast-drying (20 minutes), Refractive index close to that of glass (1.510), chemically neutral, little sensitivity to water, no reaction to staining, temperature limits: 42ºC-17ºC,not affected by ultraviolet light, non yellowing, unaffected by cold, stays optically clear for over 10 years, can be used in place of epoxy resins for mounting petrographic thin sections, used in fluorescence dyes in bones. Store at RT. MSDS

IM024 Eukitt™, mounting medium AUD144.00 /100mL stock?

PERMOUNT™ MOUNTING MEDIUM
A toluene-based synthetic resin mounting medium. The right choice for both rapid mounting and long term storage of slides. Its low viscosity allows for a thinner mounting layer offering better optical quality and bubble-free preparations.
It has a refractive index of 1.52, which is near that of a fixed protein and this helps to keep images free from distortion. Ideal for mounting coverslips to slides with thick or thin specimens. Permount preserves most biological stains with little or no fading when the slides are stored in darkness. It contains an antioxidant to prevent the formation of annular rings and its high softening point of 155^oC makes it suitable for microprojection. MSDS

IA019 Permount mounting medium AUD61.00 /100mL stock?
IA0195 Permount mounting medium AUD143.00 /500mL stock?

WAFER-MOUNT™ 559
This is available in sheet form 254x254mm. To use, remove the paper backing exposing the adhesive layer. The substrate is positioned on the film and pressed down firmly so that it adheres to the plastic film. No heating is required. The wafer mounted on the plastic film can then be held down using a vacuum manifold or mechanical means for dicing or scribing. After dicing or scribing, the parts can be separated from the wafer-mount 559 film by washing in acetone or MEK. Ideal for holding down silicon wafers, alumina, or glass substrates for scribing.

User note for Crystalbond and Wafermount

I559 Wafer-Mount™ 559 adhesive, 254 x 254mm AUD60.00 /pk/2 10% discount for 5 stock?

WAFER-MOUNT™ 562
A dry temporary adhesive film, 0.075mm thick, which can be easily cut to size with either a razor or scissor. This mount permits pre-placement of the adhesive film exactly where the user wants it. It melts at 90.5-98.9°C. After application it can be dissolved rapidly in trichloroethylene or toluene. This material is particularly useful in applications where a pre-formed adhesive film is needed to assure uniform bonding and flatness or in bonding stacks of substrates such as LCD glass slides for slicing. Sheet size 203x254mm.

User note for Crystalbond and Wafermount

I562 Wafer-Mount™ 562 adhesive, 203 x 254mm AUD145.00 /pk/10 stock?

CRYSTALBOND
A temporary adhesive. These wash-away adhesives are used as a temporary bond for holding delicate crystals, metallurgical specimens, glass components, and ceramic substrates for dicing, slicing, drilling and polishing. These materials adhere readily to metals, glass, ceramic and then can be washed, after machining away using various solvents.

User note for Crystalbond and Wafermount

Applications:
Machining or slicing single crystal metal specimens. Grinding and polishing sapphire, ceramic, optical garnets, ferrites, and LCD glass. Dicing and slicing germanium and silicone wafers in semiconductor production. Dicing and slicing alumina and beryllia substrates for IC and microelectronic production. Holding beam leads in IC devices for pull-off tests. Dicing subminiature chip capacitors and microwave IC substrates.

Crystalbond Properties:

	509	555	590
Description:	Thermo polymer	Thermo polymer	Thermo polymer
Form:	22mm dia.x182mm; stick	Rectangle stick	Rectangle stick
Softening:	71°C	52°C	125°C
Flow Point:	135°C	54°C	150°C
Viscosity at Flow Point:	6,000 cps	500 cps	9,000 cps
Colour:	Clear	White	Brown
Solvent:	Acetone or MEK	Hot Water	590-S Stripper

Crystalbond 509:
Offers excellent adhesion to metals, glass, and ceramic. This material is the best for precise high purity work since it leaves no residue after dissolving and does not clog the diamond wheel as compared to conventional waxes. Each stick weighs about 90 grams. MSDS

Crystalbond 555:
Is the same as 509 but used in applications where it is desirable to have a temporary bond which is water soluble. Each stick weighs about 90 grams.

Crystalbond 590:
Is the same as 509 but offers maximum adhesion to subminiature parts. Each stick weighs about 225 grams.

I509 Crystalbond 509, temporary adhesive AUD57.00 /stick 10% discount for 5 stock?
I555 Crystalbond 555, temporary adhesive AUD70.00 /stick stock?
I590 Crystalbond 590, temporary adhesive AUD85.00 /stick 10% discount for 2 stock?
I590S Stripper for Crystalbond 590 AUD88.00 /100g stock?

IMMERSION OILS
Cargille was first to introduce PCB-Free immersion oils in 1972. Though all immersion oils are now PCB free, no other immersion oil meets FDA criteria; and Cargille's exceeds ISO, DIN and all instrument manufacturer's standards.

Blending Oils from the Miscible Group: The Miscible Group of immersion oils is A, B, NVH and OVH. Users can easily blend any two immersion oils from the Miscible Group to obtain an immersion oil with an intermediate viscosity while maintaining the optical properties common to both. Type 37 can be blended with type B.

All Cargille immersion oils are classed as low fluorescence, however, for serious fluorescence work, types FF and DF - see below - are required.

50mL is supplied in a high density polyethylene bottle with a pointed, twist closure suited to place single drops onto slides. For refills and large users these oils are available in larger glass bottles and in the original 1 US gallon (3.785 litre) steel cans with resealable pouring spout. We stock the gallon container for type B only, but will procure other types on indent within three weeks.

For Normal Light Microscopy use Types A and B.
Elevated Temperatures (>23°C to 37°C): Use Type 37.
Inverted, Inclined, Projection, and Long Focus Intruments: Use type NVH or OVH. the greater the gap between the cover glass and objective, or between the slide and condenser, the more desirable high viscosity becomes. Their very high viscosities give excellent results for these applications.

Fluorescence Microscopy: Extremely low fluorescence is achieved by Type DF and Type HF.

Types A & B Immersion Oils
These two types practically vary only in viscosity and they are interchangeable and miscible with each other for intermediate viscosities. Produced in larger quantities than other types, Types A and B are the most economical. The deciding factor in choosing between them is the optimum viscosity for your particular application.

Type A, at 150 centistokes, reduces any tendency to trap air, especially helpful to beginning students. Air bubbles cause image degradation. Also Type A is less sticky and easier to clean up.

Type B, at 1250 cSt, is thick enough for viewing multiple slides with one application. This saves time during batch processing. Type B is the most used of these immersion oils. For a greater gap between cover glass and objective, (or condenser and slide) type B is more desirable.
Oil Type A & Oil Type B MSDS

IC113 Immersion oil type A AUD14.00 /50mL stock?
IC1132 Immersion oil type A AUD40.00 /200mL stock?
IC114 Immersion oil type B AUD9.00 /50mL stock?
IC1142 Immersion oil type B AUD23.00 /200mL stock?
IC1143 Immersion oil type B (1 US gal) AUD277.00 /3.785L stock?
IC1145 Immersion oil type B AUD50.00 /500mL stock?

HIGH VISCOSITY IMMERSION OILS FOR INVERTED MICROSCOPES
For horizontal, inverted and inclined instruments and projection equipment, Type NVH with high viscosity of 21,000cST or Type OVH with a viscosity of 46,000 cST, (about 3 times that of honey) should be used. These types combine high or very high viscosity with "normal" refractive index properties. The greater the gap between the cover glass and objective, or between the slide and condenser, the more desirable high viscosity becomes. Their very high viscosities give excellent results for these applications.
Oil Type NVH MSDS
Oil Type OVH MSDS

IC115 Immersion oil type NVH AUD12.00 /50mL stock?
IC1151 Immersion oil type NVH AUD88.00 /450mL stock?
IC1152 Immersion oil type NVH AUD38.00 /200mL stock?
IC118 Immersion oil type OVH AUD13.00 /50mL stock?
IC1182 Immersion oil type OVH AUD38.00 /200mL stock?
IC1185 Immersion oil type OVH AUD74.00 /450mL stock?

TYPE 37 HIGH VISCOSITY IMMERSION OIL
Immersion Oil Type 37 is useful for microscopic studies at body temperatures or when high room and stage temperatures may approach 37°C. Since the refractive index/ temperature relationship is inverse, increasing temperature significantly reduces the refractive index and lowers the viscosity of immersion oils. Cargille resolved this problem with Type 37 immersion oil. For any Elevated Temperatures (>23°C to 37°C) use Type 37. Users can blend for their own working temperature; blending Type B, with a viscosity of 1250 cSt at 23°C with Type 37, 1250 cSt at 37°C maintains a constant 1250 cSt viscosity and optical values and places the temperature of calibration proportionally between 23°C to 37°C.
MSDS

IC137 Immersion oil type 37 AUD16.00 /50mL stock?
IC1372 Immersion oil type 37 AUD44.00 /200mL stock?
IC1375 Immersion oil type 37 AUD84.00 /450mL stock?

IMMERSION OILS for FLUORESCENCE MICROSCOPY by Cargille
PCB-Free immersion oils. Type DF combines very low fluorescence and ideal optical properties. It is recommended where specimen fluorescence is good and highest resolution is required. Type FF does not fluoresce but its optical characteristics are not quite perfect. 50ml is supplied in a high density polyethylene bottle with a pointed, twist closure for placing single drops onto slides. For refilling it is also available in 200 and 450mL glass bottles.
DF MSDS
FF MSDS

IC1161DF Low fluorescence immersion oil type DF AUD95.00 /450mL stock?
IC1162DF Low fluorescence immersion oil type DF AUD48.00 /200mL stock?
IC116DF Low fluorescence immersion oil type DF AUD18.00 /50mL stock?
IC1171FF Fluorescence free immersion oil type FF AUD99.00 /450mL stock?
IC1172FF Fluorescence free immersion oil type FF AUD50.00 /200mL stock?
IC117FF Fluorescence free immersion oil type FF AUD17.00 /50mL stock?

REFRACTIVE INDEX LIQUID, HIGH DISPERSION
High Dispersion Liquids specially formulated for dispersion staining, focal masking, and double variation refractometry.
SERIES E: Range nD 1.500-1.640, adjustment ±0.0005.
Slightly volatile, keep tightly stoppered. Supplied with optical constants for F, D, and C lines. Recommended by prominent microscopists who have developed and published these techniques.

IC120 Refractive index liquid, Series E, nD 1.5800, 7.4mL AUD78.00 stock?