• Chinese-made coverglass on page G8
• Muscovite mica on page G9
• For easy handling of coverglass see vacuum wands on page T12
• Borosilicate glass properties

Most of our coverglass is packaged 100/box. It is the finest available, very flat but flexible, made from clear, optically superior borosilicate, hydrolytic class 1. glass. Polished clean and ready to use. Refractive index is 1.524 plus/minus 0.002 Ne (Hg 'e' line 546mm).To facilitate price comparisons with the frequently quoted but obsolete ounces (28g), we have included the net weight per box of some coverglass in the descriptions; this is based on dimensions and the specific weight of 2.52.
Coverslips packed for Shandon and Medite Cover-Slippers may be ordered on indent from a German manufacturer. Advise your requirements and we will furnish our competitive quotation.
Special orders must be for at least 10x100 slips; prices would be similar to comparable sizes below, but if they are a non standard size a surcharge may apply.

Standard thickness
No.0 ......... 0.08 - 0.12 mm
No.1 ......... 0.13 - 0.17 mm
No.1.5........ 0.16 - 0.19 mm
No.2 ......... 0.19 - 0.23 mm
No.3 ......... 0.28 - 0.32 mm
No.4 ......... 0.38 - 0.42 mm
No.5 ......... 0.50 - 0.60 mm

User note - Coverglass technical information

COVERGLASS SQUARES & RECTANGLES No.1 thickness
All our coverglass for standard size slides is of the optically preferred No.1 thickness - 0.13 to 0.17mm. European manufacture. See also two sizes of elongated cover slips for Leighton tubes on this page.
G416 is a non-standard size, therefore is more expensive.

G416 Coverglass No.1, 13 x 13mm AUD31.00 /box/200 10% discount for 5 Available
G409 Coverglass No.1, 18 x 18mm AUD5.00 /box/100 10% discount for 10 Available
G410 Coverglass No.1, 24 x 24mm AUD8.00 /box/100 10% discount for 10 Available
G414 Coverglass No.1, 24 x 40mm AUD15.00 /box/100 10% discount for 10 Available
G415 Coverglass No.1, 15 x 15mm AUD6.00 /box/100 10% discount for 10 Available
G417 Coverglass No.1, 24 x 50mm AUD13.00 /box/100 10% discount for 10 Available
G418 Coverglass No.1, 24 x 60mm AUD18.00 /box/100 10% discount for 10 Availability
G422 Coverglass No.1, 22 x 22mm AUD7.00 /box/100 10% discount for 10 Availability
G450 Coverglass No.1, 50 x 50mm AUD40.00 /box/100 10% discount for 10 Available
G470 Coverglass No.1, 48 x 70mm AUD54.00 /box/100 10% discount for 10 Available

COVERGLASS FOR COVERSLIPPING MACHINES
Thickness #1 (i . e. 0.13 - 0.17 mm) borosilicate glass of hydrolytic class 1, precisely cut in accordance with ISO 8255/1, without streaks and blisters. Packed in boxes of 100 pieces and in cartons of 10 boxes = 1000 coverslips. Specially treated surface to avoid sticking and for faster separation of the slips.

G412-2440 Coverglass for coverslipping machines, No.1, 24 x 40mm AUD70.00 /pk/1000 10% discount for 5 Available
G412-2450 Coverglass for coverslipping machines, No.1, 24 x 50mm AUD80.00 /pk/1000 10% discount for 5 Available
G412-2460 Coverglass for coverslipping machines, No.1, 24 x 60mm AUD90.00 /pk/1000 10% discount for 5 Available

COVERGLASS SQUARES No.0 thickness

G409-0 Coverglass No.0, 18 x 18mm AUD6.00 /box/100 10% discount for 10 Available

COVERGLASS RECTANGLES large & very large, No 1.5 thickness
Slightly thicker No 1.5 (0.155-0.185mm) coverglass is used for extra strength.

G418-15 Coverglass No.1.5, 24 x 60mm AUD15.00 /box/100 10% discount for 10 Available
G460-15 Coverglass No.1.5, 48 x 60mm AUD51.00 /box/100 10% discount for 10 Available
G480-15 Coverglass No.1.5, 80 x 70mm AUD89.00 /box/100 10% discount for 10 Available

COVERGLASS CIRCLES No.1 thickness

G401-06 Coverglass No.1, Ø 6mm AUD22.00 /box/200 10% discount for 5 Available
G401-10 Coverglass No.1, Ø 10mm AUD22.00 /box/200 10% discount for 5 Available
G401-12 Coverglass No.1, Ø 12mm AUD21.00 /box/200 10% discount for 5 Available
G402 Coverglass No.1, Ø 13mm AUD21.00 /box/200 10% discount for 5 Available
G403 Coverglass No.1, Ø 50mm AUD32.00 /box/100 10% discount for 10 Available
G404 Coverglass No.1, Ø 8mm AUD13.00 /box/100 10% discount for 10 Availability
G405 Coverglass No.1, Ø 18mm AUD21.00 /box/200 10% discount for 5 Available
G406 Coverglass No.1, Ø 35mm AUD22.00 /box/100 10% discount for 10 Availability
G407 Coverglass No.1, Ø 24mm AUD17.00 /box/100 10% discount for 10 Available
G408 Coverglass No.1, Ø 22mm AUD22.00 /box/200 10% discount for 5 Available
G430 Coverglass No.1, Ø 30mm AUD17.00 /box/100 10% discount for 10 Available
G442 Coverglass No.1, Ø 42mm AUD32.00 /box/100 10% discount for 10 Availability

COVERGLASS CIRCLES No.0 thickness

G400-0 Coverglass No.0, Ø 5mm AUD15.00 /box/100 10% discount for 10 Available
G402-0 Coverglass No.0, Ø 13mm AUD22.00 /box/200 10% discount for 10 Available
G404-0 Coverglass No.0, Ø 8mm AUD19.00 /box/100 10% discount for 10 Availability

COVERGLASS CIRCLES No.1.5 thickness

G420-15 Coverglass No.1.5, Ø 15mm AUD12.00 /box/100 10% discount for 10 Available
G405-15 Coverglass No.1.5, Ø 18mm AUD13.00 /box/100 10% discount for 10 Available

EXTRA THICK COVERGLASS No.5 thickness

G419-5 Coverglass No.5, Ø 19mm AUD52.00 /box/100 10% discount for 10 Available

COVERSLIPS FOR LEIGHTON TUBES
Manufactured from clear-white borosilicate glass. They offer super clarity, are perfectly flat and corrosion resistant.

G390 Coverslips No.1, 13 x 54mm for Leighton tubes L6450 AUD87.00 /box/120 Available
G395 Coverslips No.1, 10.5 x 35mm for Leighton tubes L6452 AUD71.00 /box/215 Available

QUARTZ COVERSLIP
0.17 to 0.21mm thick, 25.4mm diameter, 1 per package.
Square 25.4mm slips and 76mm x 25.4mm slides are available, but minimum order of 10 pieces please.

All our polished quartz slides and cover glasses are manufactured from G.E. 124 Fused Quartz. They are polished to a 80/50 scratch/dig. This equates to a 5 micron or better finish.
Note: ProSciTech can supply, on indent, a range of quartz products including Flasks, Beakers, Crucibles, Wool, Frits and Tubes.

Machined quartz - Optical properties
The optical properties of fused quartz let us choose between various types, because the degree of transparency defines material purity and the method of manufacture.
Certain indicators are the UV cutoff and the presence or absence of bands at 245nm and 2.73µm. The UV cutoff varies from 155 to 175nm for a 10mm thick test sample. The presence of transition metallic impurities will move the UV cutoff towards longer wavelengths.
The curves in the figure represent the average transmissions for a 10mm thick test sample for both commercial and optical grades. Fused quartz is very efficient for the transmission of infrared radiation. Infrared transmission extends out approximately 4 micrometers, with little absorption in the “water band” at 2.73µm.

User notes - Properties of fused quartz

G382 Quartz coverslip, round Ø 25.4mm AUD25.00 /each 10% discount for 10 Available
G382-SQ Quartz coverslip, square 25.4 x 25.4mm AUD25.00 /each 10% discount for 10 Available

RINZL PLASTIC COVERSLIPS
Features

• Made from clear vinyl plastic
• Unbreakable, will not scratch or cut fingers
• Used in biological and medical labs, etc.
• Thickness 0.28mm
• Refractive index of glass
• Not autofluorescent

G52-1818 Rinzl plastic coverslips, 18 x 18mm AUD15.00 /pk/100 Availability
G52-2222 Rinzl plastic coverslips, 22 x 22mm AUD15.00 /pk/100 Availability
G52-2450 Rinzl plastic coverslips, 24 x 50mm AUD15.00 /pk/100 Available

THERMANOX COVERSLIPS
Unbreakable coverslips, these have been treated for optimum cell attachment and growth. Useful in most tissue culture applications in place of glass coverslips. Thermanox coverslips have the same thickness as No.1.5 glass coverslips. They are made of a special solvent-resistant plastic material with very low gas permeability. Cell attachment and growth are comparable to polystyrene and glass. Thermanox may be used with amyl acetate, glutaraldehyde or propylene oxide for EM preparations. Also resistant to acetone, alcohol, xylene and other commonly used solvents. Can be easily cut with scissors. Supplied sterile.

User Notes

GL081 Thermanox coverslips, 22 x 60mm AUD102.00 /pk/100 10% discount for 5 Availability
GL082 Thermanox coverslips, 24 x 30mm AUD102.00 /pk/100 10% discount for 5 Available
GL083 Thermanox coverslips, Ø 13mm AUD102.00 /pk/100 10% discount for 5 Available
GL084 Thermanox coverslips, Ø 22mm AUD102.00 /pk/100 10% discount for 5 Available
GL085 Thermanox coverslips, Ø 25mm AUD102.00 /pk/100 10% discount for 5 Available

LifterSlip™ PREMIUM PRINTED COVERGLASS
LifterSlip™ offers a simple solution to the problem of hybridisation non-uniformity. An innovative raised edge design provides separation and allows for even dispersal of hybridisation solution between the DNA chip and coverslip. LifterSlips™ allow for increased data quality by eliminating gradients caused by floating standard coverslips on hybridisation solutions. The printed bars allow for easy injection of hybridisation solution under the pre-positioned coverslips. Capillary attraction ensures that LifterSlip™ stays in place. Depending on size and design configuration LifterSlip™ can hold a range of volumes.
LifterSlip™ is No.2 thickness (0.20-0.22 mm) and the bar height is 0.04-0.06 mm.

• Hybridisation & cell culture slides/vessels on H5

G481 LifterSlip™ coverglass, 18 x 18mm, 7.55µL AUD110.00 /pk/160 Available
G483 LifterSlip™ coverglass, 22 x 40mm, 20.84µL AUD110.00 /pk/59 Available
G485 LifterSlip™ coverglass, 22 x 60mm, 31.25µL AUD110.00 /pk/39 Available
G487 LifterSlip™ coverglass, 25 x 60mm, 35.83µL AUD113.00 /pk/35 Available

ROUND PHOTOETCHED COVERSLIPS
These coverslips have a grid pattern etched onto #1.5 (0.17–0.25mm) thickness cover slips. On the 25mm round slips there are 200 coded areas. Unique grid pattern, numbered 1 to 200 squares, each 500µm width. The other sizes have 520 coded squares. The appearance of the etched pattern is described with item G491. The squares are visible under brightfield and have high contrast when using phase contrast microscopy.
These slips are used to facilitate mounting of micromanipulated cells or chromosome spreads.
Reference: Lin, Lin-Fang and Frank H. Riddle 1981. Photoengraving of Cover Slips and Slides to Facilitate Mounting of Micromanipulated Cells or Chromosome Spreads, Exp. Res. 134:485-488.

G490 Round photoetched coverslips, Ø 25mm AUD346.00 /pk/25 10% discount for 5 Available

CORRELATIVE MICROSCOPY COVERSLIPS®
A unique film reticle for use in Correlative Microscopy. Designed specifically to allow identification and location of a particular area of interest under brightfield or fluorescence microscopes and then sectioning for electron microscopy.
Products developed in conjunction with Nacer Benmeradi, PhD of CNRS Toulouse and Delta Microscopies, France.

Reticle film has been thoroughly tested with cell culture

• Good growth of cell culture (equivalent to conventional media)
• Good adhesion to the substrate without cell polylysine

Physical and chemical qualities

• Resistant to normal chemicals used in electron microscopy
• No oxygen retention, compatible resin LR White
• Good optical quality in brightfield & UV fluorescence
• Excellent transparency
• Does not deform at temperatures (positive 100C and negative liquid N2)
• Rigid, does not float in the middle of culture
• Easy to handle and cut with a knife or micro-punch
• Simple sterilisation using alcohol or UV
• Detaches easily from resin after polymerisation
• Low cost

Applications
Light microscopy, fluorescence, scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-pressure cryofixation.

Cat. No.	Pattern code	Number of squares	Surface covered	Unit size of each square	Average number of cells per square unit (eg. HeLa cell)
G493-01	CMC34A	100	5x1mm²	0.01mm²	2-3
G493-05	CMC71	400	100mm²	0.25mm²	20-30
G493-10	CMC35	100	100mm²	1mm²	40-50

Correlative microscopy coverslips

G493-01 Correlative Microscopy Coverslips®, 10 x 10 grid of 0.1mm squares AUD92.00 /box/25 Available
G493-05 Correlative Microscopy Coverslips®, 20 x 20 grid of 0.5mm squares AUD83.00 /box/25 Available
G493-10 Correlative Microscopy Coverslips®, 10 x 10 grid of 1mm squares AUD83.00 /box/25 Availability

ACLAR® FILM
see Kingsley (3) for an explanation of these points:

• separates easily from epoxy
• transparent fluorinated-chlorinated thermoplastic without volatile components
• chemically inert for all practical purposes
• cells adhere to it readily and remain attached after fixation, dehydration and critical point drying or embedding
• accepts heavy metal sputter coating
• stable in SEM; melting point 202°C
• ACLAR® is as transparent as glass
• fluorescence microscopy possible since ACLAR® exhibits no detectable auto fluorescence
• can be sectioned without damaging ultramicrotomy knives
• considerably simplifies the preparation of cultured cells for all types of microscopy
• sterilisable
• gives flat sections
• smooth surface makes LM observations possible
• does not degrade under UV or gamma ray radiation
• used as an O₂ barrier when flat embedding methacrylate or acrylic resins

Description and Ordering of ACLAR® Film
The 1968 paper by E.B. Masurovsky (1) and R.P. Bunge was the first to describe and clearly demonstrate the useful chemical and physical properties of ACLAR® for both tissue culture and electron microscopy. It was first used in the space program and its properties were found to be valuable for biomedical research (personal communication, Dr. E. Masurovsky). Subsequent work, especially by Mawe (2), et al and the comprehensive ACLAR study by Kingsley and Cole (3) developed ACLAR's applications.
Because of its chemical inertness, non-stick property, glass clarity, flexibility and smooth surface ACLAR solves several problems in the processing of tissue culture cells during their epoxy embedding, sectioning and study.
ACLAR may be cut with scissors or blades and does not damage microtomy knives. It is unsurpassed as a moisture barrier, it is transparent to UV and is plasticiser and stabiliser free. It is nonflammable, non-ageing and has a low dielectric constant and dissipation. It is high in dielectric strength. ACLAR can be sterilised.
Fresh material may be cut on a vibrating tissue slicer, treated with HRP and then placed on ACLAR cut into a slide shape; observed under LM and if satisfactory the process for TEM may be completed.

References, ACLAR® Film
1. Masurovsky EB, Bunge RP: Cover slips for long-term nerve tissue culture. Stain Technology, 43, 3, 161 - 165 (1968)
2. Mawe GM, Bresnahan JC, Beattie, , MS: Ultrastructure of HRP-labelled neurons: a comparison of two sensitive techniques. Brain Research Bulletin, 10, 551 (1983)
3. Kingsley RE, Cole NL: Preparation of cultured mammalian cells for transmission and scanning electron microscopy using Aclar film. J of Electron Microscopy Technique, 10, 77-85 (1988)

ACLAR® is supplied in packets of ten sheets.
An 11mm disk punch is available.

GL105 ACLAR® plastic film 203 x 318mm, thickness 0.198mm AUD170.00 /pk/10 Available