Lowicryl resins are highly cross-linked acrylate-based embedding resins, designed for use over a wide range of embedding conditions. These resins provide low viscosity at low temperatures. The K4M kit is usable to -35°C and is a polar (hydrophilic) medium, while the HM20 kit can be used down to -70°C and is nonpolar (hydrophobic). Lowicryl's K11M and HM23 have properties similar to K4M and HM20, but can be used at temperatures 20 degrees lower than others: K11M at -60°C, HM23 at least -80°C. All of these resins are photopolymerised by long wavelength (360nm) ultraviolet light. Since the initiation of the polymerisation is largely independent of temperatures, blocks may be polymerised at the same temperatures used for infiltration. The resin may also be chemically polymerised at 60°C.
Lowicryl MonoStep Single Component Embedding Media
The same as our Lowicryl complete kits but this new formulation allows you one stop embedding without all of the cumbersome preparation and mixing. This pre-mixed, ready to use resin, saves you time and minimises chemical contact. MonoStep Lowicryl is ideal for use in immunohistochemistry and immunolabeling. Store at room temperature.
C430 MSDS
C435 MSDS
C440 MSDS
C445 MSDS
C450 MSDS
C460 MSDS
User Notes
C440 contains: 3 x 225mL Monomer E, 1 x 130g Crosslinker D, 4.8g Initiator C
C430 Lowicryl K4M ask
C435 Lowicryl K4M monostep (polar) ask
C460 Lowicryl HM23 ask
C440 Lowicryl HM20 embedding kit ask Availability
C445 Lowicryl HM20 polar embedding medium MonoStep AUD429.00 /225g Availability
C450 Lowicryl K11M kit AUD801.00 /kit Available
Consists of:
- 500mL LR Gold Resin (C028)
- 100g Polyvinyl pyrolidone (C029)
- 50g Benzil (C030)
C036 LR Gold embedding kit AUD292.00
LR GOLD RESIN
The London Resin Company's acrylic "LR Gold" is the resin of choice when the antigenicity of the tissue is quite fragile. LR Gold is cured by the blue fraction of white light and this is possible at low temperatures. LR Gold is especially designed for low temperature (-25°C) embedding of fresh or lightly aldehyde-fixed tissue. Choice of initiator will determine polymerisation method - UV to intense white light. A PVP concentration above 20% may improve EM morphology but can also result in very high viscosities at low temperatures. LR Gold is non-toxic and has a low viscosity. MSDS
User Notes
For "cold curing" with light of non-osmicated tissue.
MSDS
User Notes
C027 LR White accelerator AUD30.00 /10mL Available
For cold, light cured resin embedded specimens. Consists of:
- 500mL LR White (C023 or C024)
- 10mL LR White accelerator (C027)
- 9.9g Benzoyl peroxide powder (catalyst) MSDS
User Notes
C025 LR White embedding kit, medium AUD249.00 /kit
C026 LR White embedding kit, hard AUD252.00 /kit
A very low viscosity (8cps), non-toxic resin, suitable for both light and electron microscopy. LR White is a polar monomer polyhydroxylated achromatic acrylic resin. Curable by heat or light. Sections of polymerised LR White resin are hydrophilic. This characteristic allows immunocytochemistry reagents and stains to easily penetrate the sections. Etching of sections is not necessary. LR White sections show minimal non-specific staining.
Its low viscosity makes it ideal for infiltrating decalcified bone and teeth as well as plant tissue. It is available in medium and hard grade.
Distance, time and high temperatures during shipping from the manufacturer would age catalysed resin too much, we purchase and ship LR White uncatalysed, but the material is supplied with catalyst (benzoyl peroxide) and detailed instructions, but read in the linked User Notes how to catalyse without ruining the whole bottle. Catalysed LR White keeps refrigerated for over one year and embedded specimens can be cured using heat only. For cold curing with light, accelerator must be added.
Newman, G.R. Use and abuse of LR White. Histochem.J. 19:118 (1987)
Newman, G.R. LR White embedding medium for colloidal gold method. In: Colloidal Gold: Principles, Methods, and Applications, Vol.2 (Hayat, M.A., Ed.). Academic Press, San Diego and London (1989).
Note: Minor acid number variation of some resins results in a colour change when the mixture is made up: from clear to amber to pink. This does not affect resin performance.
Benzoyl peroxide MSDS
User Notes Adding catalyst
User Notes Electron microscopy
User Notes Hard tissue
User Notes Immunohistochemistry
User Notes Light microscopy
C023 LR White resin, medium grade AUD215.00 /500mL Available
C024 LR White resin, hard grade AUD220.00 /500g Available
C13H9Li2N5O9 S2 F.W. 457.25
A technique producing an electron dense osmiophilic polymer when lucifer yellow is injected into cells. Lucifer yellow can also be viewed in living cells as a dye marker and is more sensitive than any other dye. Cell Tissue Res., 204,37 (1979). J.Histochem. Cytochem., 30,189 (1982). Science, 216,943 (1982); 214,801 (1981) 225,851 (1984). Cell, 14,741 (1978). Fluorescent dye: Nature 292,17 (1981) MSDS
C122 Lucifer Yellow AUD175.00 /25mg Available
MgSO4.7H2O CAS #100034-99-8
M = 246.48g/mol
AR grade
C230 Magnesium sulphate AUD52.00 /500g Availability
MALEIC ACID
F.W. 116.08 Crystalline, repurified, A.C.S. Assay 99.5% toxilic acid; cis-butenediodic acid. M.P. 132-134°C. MSDS
This stain is sometimes favoured over Jenner's and it too is a combination of Eosin Y and Methylene Blue. Jenner's versions required aging, but this chemically polychromed Romanowsky type stain may be used immediately after preparation.
The Biological Staining Commission does not certify May-Gruenwald. Of course our stain performs as intended. The powder is dark green and soluble in water and methanol. MSDS
C520 May-Gruenwald stain AUD18.00 /25g Stock?
C5201 May-Gruenwald stain AUD38.00 /100g Stock?
C5205 May-Gruenwald stain AUD132.00 /500g 5% discount for 2 Available
For use as Aniline Blue in Mallory's connective tissue stain. MSDS
C123 Methyl Blue, certified, C.I.42780 AUD254.00 /25g Availability
F.W. 458.48 Also called Basic Blue 20.
Used as a counter-stain in Almann's aniline acid fuchsin techniques for mitochondria in Kurnick's Methyl Green-Pyonin stain for nuclei and cytoplasmic granules of liver cells.
Boyd, T.J.B., and Mitchell, H.R.,
Marker for DNS at acid and natural conditions. Analy.Biochem., 13,28 (1965)
MSDS
C125 Methyl Green, certified, C.I. 42590 AUD50.00 /25g Stock?
C1251 Methyl Green, certified, C.I. 42590 AUD158.00 /100g Stock?
C1255 Methyl Green, certified, C.I. 42590 AUD646.00 /500g Available
Chemical Formula:C5H8O2
MSDS
C651 Methyl methacrylate AUD91.00 /225mL Available
F.W. 327.34. Also called Acid Orange 52.
Used as a stain for pituitary acidophils with the Wilson-Ezrin method, in the Kreyberg stain and in Flemming's triple stain on plant material. MSDS
C118 Methyl Orange, C.I. 13025 AUD15.00 /25g Stock?
C1181 Methyl Orange, C.I. 13025 AUD26.00 /100g Stock?
C1185 Methyl Orange, C.I. 13025 AUD80.00 /500g Available
Free acid, high purity, additive to various media for colour indication of pH. MSDS
C512 Methyl Red, C.I. 13020 AUD17.00 /25g Stock?
C5121 Methyl Red, C.I. 13020 AUD32.00 /100g Stock?
C5125 Methyl Red, C.I. 13020 AUD104.00 /500g 5% discount for 2 Available
Chemical Formula: C15H14N3NaO2, F.W.291.29, CAS No. 845-10-3
Technical grade, for industrial use.
MSDS
C5131 Methyl Red, sodium salt ACS AUD30.00 /100g Stock?
C5135 Methyl Red, sodium salt ACS AUD97.00 /500g Stock?
F.W. 292.06.
Used with Gentian Violet and Bismarck Brown Y for staining metachromatic granules of diphtheria organisms. Minimum dye content 75%.
C126 Methyl Violet, certified, C.I. 42535 AUD15.00 /25g Stock?
C1261 Methyl Violet, certified, C.I. 42535 AUD26.00 /100g Stock?
C1265 Methyl Violet, certified, C.I. 42535 AUD76.00 /500g Available
F.W. 319.86+aq. Used to distinguish autonomic nerves.
Richardson K.C., Anat.Red., 164,231 (1969). General stain for animals, histology. Dougherty, M.M., and King, J.S., A simple, rapid staining procedure for methacrylate embedded tissue sections using chromotrope 2R and methylene blue. Stain Tech., 59,149 (1984). Shroeder H.E., Rossinsky, K., and Muller, W., An established routine method for differential staining of epoxy-embedded tissue sections. Microsc.Acta 83,111 (1980)MSDS
C124 Methylene Blue, certified, C.I. 52015 AUD16.00 /25g Stock?
C1241 Methylene Blue, certified, C.I. 52015 AUD31.00 /100g Stock?
C1245 Methylene Blue, certified, C.I. 52015 AUD98.00 /500g Available
Molecular sieve type 4A
For drying ethanol, acetone and ammonia. In pellets form 2.5x5mm. The pellets may be dried and re-used like silica gel. Do not place solvent saturated pellets into an oven!
C830 Molecular sieve type 4A AUD138.00 /250g Availability
For use in supra-vital staining of living blood cells. Minimum dye content 50%. MSDS
C127 Neutral Red, certified, C.I. 50040 AUD20.00 /25g Stock?
C1271 Neutral Red, certified, C.I. 50040 AUD45.00 /100g Stock?
C1275 Neutral Red, certified, C.I. 50040 AUD160.00 /500g Available
Water soluble. For negative staining of bacteria.
With Basic Fuchsin, staining of bacteria spores. Dorner, Le Lait, 6,8 (1926)
MSDS
C128 Nigrosin, w/s, C.I. 50420 AUD14.00 /25g Stock?
C1281 Nigrosin, w/s, C.I. 50420 AUD24.00 /100g Stock?
C1285 Nigrosin, w/s, C.I. 50420 AUD69.00 /500g Available
CAS 3625-57-8; C40H40N6O6S; FW 732.84
Note that Nile Blue A, sulphate and Nile Blue, chloride have the same CI number; they act and stain similarly. Differentiates melanins and lipofuscins; used in fat staining. Lillie, Stain Tech., 31,151 (1956). J.Histochem., 4,377 (1956). Z. Analyt. Chem.,260,290 (1972). For staining bone sections. Cytologie,11,261 (1940) MSDS
C129 Nile Blue A, sulphate, certified, C.I. 51180 AUD40.00 /25g Stock?
C1291 Nile Blue A, sulphate, certified, C.I. 51180 AUD112.00 /100g Stock?
C1295 Nile Blue A, sulphate, certified, C.I. 51180 AUD485.00 /500g Available
(The trademark NADIC is owned by another company).
F.W. 178.19, CAS: 25134-21-8. Epoxy hardener. B.P. 135°C
MSDS User Notes
C046 NMA AUD106.00 /450mL Available
